α-甘草酸对豚鼠肾11β-羟基类固醇脱氢酶活性的影响  

Effects of α-glycyrrhizic acid on 11β-hydroxysteroid dehydroxydrogenase from Hartley guinea pig kidney

作　　者：俞进[1] 林康[2] 楼宜嘉[1] 

机构地区：[1]浙江大学药学院,浙江杭州310006 [2]杭州市中医院,浙江杭州310007

出　　处：《中国现代应用药学》2003年第3期181-184,共4页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的　探讨α 甘草酸对豚鼠肾脏 11β 羟基类固醇脱氢酶 (11β OHSD2 )的抑制作用。方法　运用微粒体酶分析技术 ,观察α 甘草酸减小微粒体酶对底物皮质醇的转化率 ,并测定酶的动力学常数。结果　α 甘草酸能够减小微粒体酶对底物皮质醇的转化率 ,和阴性对照、β 甘草酸相比 ,差异均有显著性意义 ;动力学常数Vmax=15 .2 5nmol/mg .h ,Km=1.31μmol/L ,Ki=(2 0 9.2± 17.1) μmol/L。 结论　α 甘草酸能够抑制豚鼠肾脏 11β OHSD2 ,但和 β 甘草酸相比 。OBJECTIVE To demonstrate the inhibitory effects of α glycyrrhizic acid on 11β hydroxysteroid dehydrogenase from Hartley guinea pig kidney. METHOD Using microsomes from the kidney cortex,the reduction of transform rate of cortisol by α glycyrrhizic acid was observed and kinetic constants were determined.RESULTS Compared with negative group and β glycyrrhizic acid, α glycyrrhizic acid reduced transform rate of cortisol with a Ki of 209.2μmol/L±17.1μmol/L,V max =15.25 nmol/ mg.h,K m=1.31μmol/L.CONCLUSION Compared with β glycyrrhizic acid,α glycyrrhizic acid can inhibite the 11β hydroxysteroid dehydrogenase from Hartley guinea pig kidney,but was less potent than β glycyrrhizic acid.

关 键 词：Α-甘草酸 肾脏 11Β-羟基类固醇脱氢酶 微粒体酶 

分 类 号：R284[医药卫生—中药学] R963[医药卫生—中医学]