川芎药材活性部位的高效液相色谱指纹图谱定性分析方法  

HPLC-fingerprint analysis of the active part of Radix Ligusticum Chuangxiong Hort

作　　者：王嗣岑[1] 朱丽华[1] 贺浪冲[1] 

机构地区：[1]西安交通大学药学院仪器分析中心,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2003年第3期221-223,236,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目 (No .2 0 0 75 0 2 0 )

摘　　要：目的　建立川芎药材活性部位的指纹图谱 ,为其定性鉴别提供依据。方法　川芎药材经乙醇超声提取后 ,用醋酸乙酯萃取 ,采用ODS柱为分析柱 ,以甲醇 水 冰醋酸 (30∶70∶0 .5 )为流动相 ,在 2 76nm条件下进行分析 ,建立其指纹图谱。并对 7种不同来源的川芎药材进行高效液相色谱指纹图谱定性分析比较。结果　本研究建立的分析方法的精密度、重现性较好 ,以阿魏酸峰计 ,RSD分别为 1.6 8%和 1.0 4 % ;不同川芎药材指纹图谱中主要峰群的整体图貌基本一致 ,但各成分含量的相对比值有所不同 ,不同来源的川芎药材共有峰面积的比值及共有峰面积和均有一定的差异。结论　HPLC指纹图谱分析法可简便。Objective To establish a HPLC fingerprint pattern (HPLC FPS) for the active part of Radix Ligusticum Chuangxiong Hort. and make a comparative study of HPLC FPS of seven kinds of Radix Ligusticum Chuangxiong Hort. from different sources. Methods The samples were extracted with ethanol by supersonic technique for 20?min, and extracted with ethyl acetate. The ODS column was used as analytical column, the mobile phase consisted of methanol water acetic acid (30∶70∶0.5), and the flow rate was 1?mL·min -1 with UV detection at 276?nm. Results The method was proven to be plecise with good reproducibility and the RSD was 1.68% and 1.04% using ferulic acid as the standard component, respectively. The spectrum of the chromatograms was similar, but the ratio of common peaks' area of different samples was different. Conclusion This method can be used to differentiate conveniently and rapidly Radix Ligusticum Chuangxiong Hort. from different sources.

关 键 词：川芎 中药材 活性部位 高效液相色谱 指纹图谱 定性分析 四物汤 

分 类 号：R282.71[医药卫生—中药学]