肺炎支原体下呼吸道感染患儿外周血辅助性T细胞亚群Th1/Th2及B细胞的研究  

The study of PBMCs T-helper cell subpopulation Th1/Th2 and B cells for children with MP LRTIs

作　　者：史瑞明[1] 雷春莲[1] 包瑛[1] 罗树舫[1] 

机构地区：[1]西安交通大学第一医院儿科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2003年第3期234-236,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的　探讨辅助性T淋巴细胞亚群Th1/Th2及B细胞在肺炎支原体 (MP)下呼吸道感染患儿免疫学发病机制中的作用。方法　应用流式细胞仪单克隆抗体免疫荧光标记法 ,对 30例MP下呼吸道感染患儿的外周血B淋巴细胞CD19+ 及辅助性T淋巴细胞Th1亚群CD4 + CD4 5RA+ 、Th2亚群CD4 + CD4 5RO+ 的表达情况进行研究。结果　MP下呼吸道感染患儿外周血B淋巴细胞CD19+ 表达明显升高。辅助性T淋巴细胞Th1亚群CD4 +CD4 5RA+ 表达明显升高 ,Th2亚群CD4 + CD4 5RO+ 表达无显著性变化。CD4 + CD4 5RA+ /CD4 + CD4 5RO+ 明显升高。结论　MP下呼吸道感染患儿存在多种免疫失衡和紊乱情况 ,主要表现为Th1细胞为主的免疫反应及B淋巴细胞过度增殖 ,符合自身免疫性疾病的免疫病理基本特征 。Objective To study the effect of T helper cells CD4 + subpopulation Th1/Th2 and B cells in PBMCs for children with mycoplasma pneumoniae (MP) lower respiratory tract infections (LRTIs). Methods CD4 +CD45RA +, CD4 +CD45RO + and CD19 + in PBMCs were determined by flow cytometry analysis in 30 children with MP LRTIs, and then compared with those of control group. Results In MP(+) children CD19 + cells increased greatly ( P < 0.05 ). There was no difference in CD4 + cells between subject group and control group ( P > 0.05 ). CD4 +CD45RA + cells increased greatly( P < 0.01 ) while CD4 +CD45RO + cells did not increased significantly ( P > 0.05 ). The ratio of CD4 +CD45RA +/CD4 +CD45RO + increased remarkably ( P < 0.01 ). Conclusion In the PBMCs of MP LRTIs children, there is immune disorder. B cells increased obviously, and Th1/Th2 imbalance develops. It shows a predominant Th1 like immuno reaction in MP LRTIs, which suggests MP LRTIs have some association with autoimmune diseases.

关 键 词：肺炎支原体感染 下呼吸道感染 小儿 外周血 B淋巴细胞 自身免疫性疾病 TH1细胞 TH2细胞 

分 类 号：R725.6[医药卫生—儿科]