一种中间代谢途径代谢通量的定量分析方法  

A Quantitative Analysis of Intracellular Metabolic Fluxes for Central Metabolism

作　　者：张慧敏[1] 朱江峰[2] 清水和幸[2] 姚善泾[1] 

机构地区：[1]浙江大学化学工程与生物工程学系,杭州310027 [2]九州工业大学生物系统工学系

出　　处：《生物化学与生物物理进展》2003年第2期301-307,共7页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目 (2 0 2 760 65 )~~

摘　　要：以13 C标记的碳源 ,用二维核磁共振技术 (1H 13 C ,HMQC)测定代谢中产生的氨基酸标记模式 ,研究对中间代谢途径胞内代谢通量进行定量分析的方法 .通过开发软件包 ,改进同位素分布的数学模型 ,提出了反应映射矩阵 (RMM )等概念 .由简化算法 ,提高程序的执行效率 ,建立了定量分析胞内代谢通量的平台 .代谢模型涉及了糖酵解途径、磷酸戊糖途径、三羧酸循环、几种回补反应。A scheme of investigating the intracellular metabolic fluxes by isotope C-13 labeling experiments and analyzing the labeling patterns of cellular amino acids by two-dimensional [H-1-C-13] nuclear magnetic resonance spectroscopy was developed. The software package was constructed with a comprehensive and improved isotopomer model, which introduce the concept of Reaction Mapping Matrices (RMM). Thus with a simplified algorithm, the platform is established for quantitating the intracellular flux distribution and investigating the cellular metabolism more efficiently. The metabolic model includes the Embden-Meyerhof-Parnas, the pentose phosphate pathway, the tricarboxylic acid cycle, anaplerotic reaction sequences, some fermentative pathway and pathways involved in amino acid synthesis.

关 键 词：中间代谢途径 代谢通量 定量分析 同位素分布模型 二维核磁共振技术 

分 类 号：Q503[生物学—生物化学] Q591