四种园蛛同工酶的比较研究  

Studies on Isozyme of Four Species of the Genus Araneus (Araneae: Araneidae)

作　　者：王晓霞[1] 彭宇[1] 刘凤想[1] 黄静[1] 陈婧[2] 

机构地区：[1]湖北大学生命科学学院,武汉430062 [2]南京大学数学系,南京210093

出　　处：《蛛形学报》2003年第1期38-41,共4页Acta Arachnologica Sinica

基　　金：国家自然科学基金(30270895);湖北省教育厅基金(2001B01005)

摘　　要：本文采用聚丙烯酰胺凝胶电泳的方法,对叶斑园蛛、角类肥蛛(黑、白2种体色)、大腹园蛛、黑斑园蛛4种园蛛科蜘蛛的酯酶(EST)同工酶、乳酸脱氢酶(LDH)同工酶、超氧化物歧化酶(SOD)同工酶进行了比较研究。结果表明,4种园蛛的EST、LDH具有明显的种族特异性,而SOD在种间基本没有差别。不同种蜘蛛都有各自的EST、LDH同工酶谱型,可以用它们作为识别物种的附加指标。Polyacrylamide gel electrophoresis had been used to study esterase(EST) isozyme, lactate dehydrogenase (LDH) isozyme, superoxide dismutase (SOD) isozyme variations in four species of araneidae, Araneus sia Strand, A.cornutus, A. ventricosus and A.mitificus. The results showed that EST isozyme and LDH isozyme were obvious differences between the species, but there were no differences between the four species in SOD isozyme. Different kinds of spiders had different EST isozyme and LDH isozyme patterns, which could be used to take as distinguishing species' appended targets.

关 键 词：园蛛 同工酶 叶斑园蛛 角类肥蛛 大腹园蛛 黑斑园蛛 酯酶 乳酸脱氢酶 超氧化物歧化酶 

分 类 号：Q55[生物学—生物化学]