植物基因打靶技术  

Gene Targeting in Plants

作　　者：杨瑞仪[1] 曾庆平[1] 

机构地区：[1]广州中医药大学热带医学研究所,广州510405

出　　处：《中国生物工程杂志》2003年第5期69-73,共5页China Biotechnology

基　　金：国家自然科学基金资助项目 ( 30 2 715 91);广东省自然科学基金资助项目 ( 0 2 0 799)

摘　　要：基因打靶是反向遗传学的基础工具 ,它通过同源重组置换染色体内的基因用于复杂基因组的基因功能分析。但是 ,在植物中 ,外源DNA的插入主要是非序列依赖的非同源末端连接方式 ,基因打靶频率很低 ,只有 1 0 -5～ 1 0 -4 的水平。综述了近年来为了提高植物基因打靶频率 。The chromosomal gene targeting via the homologous recombination is an essential tool of reverse genetics as applied for the functional assay of genes within complex genomes.However,the foreign DNA integration in plants mainly through non-homologous end joining in a sequence-independent manner,and gene targeting frequencies are as low as about 10 -5 to 10 -4.Tremendous efforts have been undertaken to improve gene targeting efficiency in plants,and a number of approaches are recently developed and they might indeed lead to a feasible gene targeting technique in the near future.The latest progress on gene targeting in plants is reviewed.;

关 键 词：植物 基因打靶 同源重组 非同源末端连接 

分 类 号：Q943.2[生物学—植物学]