声波刺激对猕猴桃愈伤组织ATP含量的影响  

Effects of Sound Stimulation on ATP Content of Actinidia chinensis Callus

作　　者：阳小成[1] 王伯初[2] 段传人[2] 贾毅[2] 

机构地区：[1]华南师范大学生命科学学院,广州510631 [2]重庆大学生物工程学院生物力学与组织工程教育部重点实验室,重庆400044

出　　处：《中国生物工程杂志》2003年第5期95-97,共3页China Biotechnology

基　　金：国家自然科学基金资助项目 ( 39770 2 0 6)

摘　　要：采用单因子实验设计 ,分别先固定作为交变应力的声波的频率或强度这两个参数中的一个 ,而改变另一个指标的值 ,用声波刺激木本植物中华猕猴桃 (Actinidiachinensis)茎段的愈伤组织 ,并测定对比声波处理前后其ATP含量及变化情况。实验结果表明 ,声波对猕猴桃愈伤组织ATP的含量有着比较明显的增强或抑制的双重效应 ,适度的声场刺激将有利于提高植物的能量代谢水平 ,其中 ,最适的声波频率为 1 0 0 0Hz,最适声强为 1 0Using callus cultured from stalks of Actinidia chinensis,this study dealed with the effects of sound stimulation on plant cell energy metabolism i.e.content of ATP.The results indicated that there occurs bidirectional effects of ATP content on sound field stimulation.The sound intensity of about 100 dB and sound frequency of approximate 1000 Hz are optimal external stresses for energy metabolism of A.chinensis.The experiment showed that moderate sound field would be advantageous to energy metabolism and development of woody plant.The mechanisms of ATP content effects of sound stimulation on A.chinensis callus was comprehensively discussed in the light of cytobiology and molecular biology.While the essential mechanisms of biological effects of environmental stresses remains further research.;

关 键 词：中华甜猴桃 声波刺激 ATP 愈伤组织 

分 类 号：S663.4[农业科学—果树学]