机构地区:[1]第四军医大学西京医院烧伤科
出 处:《中华烧伤杂志》2003年第3期155-158,共4页Chinese Journal of Burns
基 金:国家自然科学基金资助项目 (3 90 70 82 2 )
摘 要:目的 观察不同方法制备的异种脱细胞真皮基质 (Xeno -ADM )埋植物的抗原性差异。 方法 将猪断层中厚皮片采用胰蛋白酶 +tritonX 10 0等处理 ,制成Xeno -ADM ,分为戊二醛交联的Xeno ADM1组、网状交联的Xeno ADM2 组、未交联的Xeno ADM3 组、动物先用Xeno ADM蛋白致敏后再移植交联的Xeno ADM4组和未交联的异体兔Allo ADM对照组。分别将移植物埋植于 5组共计 2 5只日本白兔的耳部和背部皮下 ,术后 2~ 32周检测兔血清抗ADMs抗体效价 ,并观察移植物大体和组织学变化。 结果 Xeno ADM4埋植物组血清抗Xeno ADM蛋白抗体效价最高 ;排除致敏因素的影响 ,Xeno ADM各组血清抗体滴度依次为 :Xeno ADM3 组 >Xeno ADM2 组 >Xeno ADM1组 (P<0 .0 5~ 0 .0 0 1) ;在Allo ADM组中 ,有 4 0 %的血清样本呈现出抗Allo ADM蛋白抗体阳性。组织学上 ,Xeno ADM移植部位有激烈而持久的炎症反应 ,明显强于Allo ADM异体埋植对照组 ;ADM被降解吸收的程度依次为 :Xeno ADM3 >Xeno ADM2 >Xeno ADM4>Xeno ADM1>Allo ADM (P <0 .0 5~ 0 .0 0 1) ,在交联型ADM组易产生异物巨细胞反应。 结论 Xeno ADM的免疫原性强于Allo ADM ,诱导宿主产生IgG限制的免疫反应 ;大张变性的ADM埋植物能降低抗原 抗体反应 。Objective To investigate the difference of the antigenicity of xenogenic acellular dermal matrix (ADM) implants prepared by different methods. Methods The split-thickness skin sheet from swine was processed by trypsin and Triton X-100 to make xeno-ADM. Twenty-five Japanese white rabbits were divided into 5 groups,i.e.xeno-ADM_1 (conjugated with glutaradehyde),xeno-ADM_2(conjugated with network) and xeno-ADM_3 (no conjugation,as control),in which the ADMs were and xeno-ADM_4(conjugated) and allo-ADM (no conjugated as control),in which the ADMs were embeded into the subcutaneous place of rabbit ear and back after that the rabbits were pre-sensitized by xeno-ADM soluble protein antigen injections.The titers of anti ADMs antibody in rabbit serum were monitored during 2~32 post-operative weeks and the histological changes of the embeded ADMs were observed grossly and microscopically. ResultsThe serum titers of anti-xeno-ADM in xeno-ADM_4 group was the highest. Whereas regardless of the sensitizing effects, the titers in all groups ranged as follows: xeno-ADM_3>xeno-ADM_2>xeno-ADM_1 ( P <0 05-0.01). About 40% serum samples in allo-ADM group exhibited positive anti-allo-ADM protein antibodies. Histologically, Evident and lasting inflammatory reaction could be found in the xeno-ADM grafting sites, which was much stronger than that in allo-ADM group. The degradation and absorption gradient of ADM was ranked as follow: xeno-ADM_3>xeno-ADM_2>xeno-ADM_4>xeno-ADM_1>Allo-ADM. Foreign body megalocytic reaction might evoke in the surrounding of conjugated ADM. Conclusion The immunogenicity in xeno-ADM was stronger than that in allo-ADM, which could induce the host to develop immune reaction restricted by IgG. Large sheets of degenerated ADM implants could lower down the antigen-antibody reaction and ameliorate the structural destroying and degeneration absorption of ADM induced by inflammatory immune reaction.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...