人外周血树突状细胞的诱导及其对自身T细胞的激发作用  

The Induction of Dendritic Cell from Human Peripheral Blood and Its Effect on Activation towards T Cell

作　　者：宋军[1] 谢炜[1] 

机构地区：[1]苏州大学医学院生理学教研室,江苏苏州215007

出　　处：《郴州医学高等专科学校学报》2003年第2期6-8,共3页

摘　　要：目的　体外诱导和扩增树突状细胞 (DC)并分析其对自身T细胞的激发作用。方法　正常人外周血单个核细胞经贴壁去除悬浮细胞 ,加入细胞因子 (IL - 4、GM -CSF和TNF -α)培养 8天。用流式细胞仪分析细胞表型 ,用ELISA测定培养上清中IL - 12的含量 ,将诱导的树突状细胞与自身T细胞混合培养用3H -TdR法测定细胞的增殖指数。结果　正常人外周血诱导的树突状细胞高表达分化抗原CD1a(89.1% )、CD4 0 (99.8% )、CD80 (95 .1% )、CD83(4 5 .7% )、HLA -DR(97.6 % ) ,同时所获的DC能分泌IL - 12和有效地激活自身T细胞 ,并促其增殖。结论　正常人外周血的单个核细胞经细胞因子的序贯培养 ,可获得高纯度。Objective To induce and proliferate dendritic cells(DC)from human peripheral blood mononuclear cells (PBMCs) in vitro and analyze its effect on activating T cell.Methods PBMC were cultured for 2 hours and then the floationg cells were removed.Cytokines including IL-4?GM-CSF and TNF-α were added into the fresh medium. After 8 days culture, phenotypes were analyzed by FACS.The level of IL-12 in the supernant was detected by ELISA. The induced DCs were also co-cultured with its T cells derived from floationg cells. Its stimulating index was detected by 3H-TdR assay. Results The induced DC highly expressed differential antigens(CDla:89.1%?CD40:99.8%?CD80:95.1%?CD83:45.7%?HLA-DR:97.6%),and it secreted IL-12 and effectively stimulated its T cells to proliferate.Conclusions DCs with high purity and special function can be generated through PBMC from human peripheral blood cultured in the medium containing cytokines

关 键 词：细胞因子 树突状细胞 表型 激发作用 

分 类 号：R392.12[医药卫生—免疫学]