肾怡对狼疮小鼠免疫器官内Th1/Th2细胞比例的调节作用  

Effect of SHEN-YI,a Chinese herbal formula on ratio of Th1/Th2 cells within immune organs of MRL/lpr lupus mice

作　　者：李非[1] 陈香美[1] 傅博[1] 候剀[1] 

机构地区：[1]解放军总医院肾科,北京100853

出　　处：《中国免疫学杂志》2003年第6期393-396,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金重点项目资助 ( 3 993 0 2 3 0 ) ;国家自然科学基金创新研究群体基金资助 ( 3 0 12 10 0 5 )

摘　　要：目的 :探讨复方中药肾怡对MRL lpr小鼠免疫器官内Th1 Th2细胞比例的调节作用。方法 :将MRL lpr狼疮小鼠随机分成对照组和中药治疗组 ,从 12周观察到 2 8周后于实验终点处死小鼠。通过流式细胞术分别检测胸腺和脾脏中CD4 + CD30 - (Th1)、CD4 + CD30 + (Th2 )淋巴细胞所占百分比 ,进一步对脾细胞进行CD4 + T淋巴细胞分选 ,并于体外利用PHA P进行刺激后 ,通过RT PCR方法检测IL 4 (Th2细胞因子 )和IFN γ(Th1细胞因子 )的表达丰度 ,比较二者的比值在对照组和中药治疗组之间的差别。结果 :胸腺中几乎检测不到CD4 + CD30 + T淋巴细胞 ;脾脏中虽可检测到CD4 + CD30 + T淋巴细胞 ,但在未加入PHA P的条件下 ,CD4 + CD30 + T淋巴细胞在对照组和中药治疗组中所占的比例没有显著差异 ;经PHAP刺激后对照组和治疗组脾细胞中CD4 + CD30 + T淋巴细胞所占百分比均明显增加 ,但治疗组中CD4 + CD30 + T淋巴细胞所占百分比的增加程度明显低于对照组 ;将脾细胞中CD4 + T淋巴细胞分选出来后进行RT PCR检测 ,结果显示中药治疗组CD4 + T淋巴细胞所表达IL 4 IFN γ(Th2 Th1)比值明显低于对照组。结论 :复方中药肾怡能够纠正MRL lpr狼疮小鼠脾脏中CD4 + T淋巴细胞在PHA P刺激的条件下朝向Th2过度分化的倾向性。Objective:To define the effects of SHEN YI,a Chinese herbal formula on ratio of Th1/Th2 cells within immune organs of MRL/lpr mice Methods:MRL/lpr mice were administered daily with the Chinese herbal formula from the age of 12 weeks and both the treatment and control groups were killed at the age of 28 weeks The percentage of CD4 +CD30 -(Th1),CD4 +CD30 +(Th2)cells within thymus and spleen were determined in vitro by FACScan with or without PHA P stimulation The CD4 + T cells selected from splenocytes were stimulated with PHA P, then, RT PCR was performed to detect IL 4/IFN γ mRNA expressed by the selected cells In addition, the ratio of the two cytokines expressed by the CD4 + T cells from the control and treatment groups was calculated respectively Results:CD4 +CD30 + T cells nearly can't be detected within Thymus Without PHA P stimulation, there was no difference in percentage of CD4 +CD30 + T cells between the control and treatment groups After stimulation with PHA P, FACScan showed an increment in the percentage of CD4 +CD30 + T cells both in the control and treatment groups, however, the extent of expansion of CD4 +CD30 + T cells percentage in treatment group was lower than it in the control one RT PCR showed a reduction in the ratio of IL 4/IFN γ mRNA in the treatment group compared to it in the control one Conclusion:The Chinese herbal formula can inhibit Th cells to differentiate into Th2 cells in the spleen under the stimulation of PHA P

关 键 词：肾怡 狼疮小鼠 免疫器官 TH1/TH2细胞 调节作用 

分 类 号：R692[医药卫生—泌尿科学]