参杞合剂对荷瘤小鼠免疫功能及细胞周期的影响  

Experimental study on anti-tumor effect with Shenqi compound recipe

作　　者：李红喆[1] 范晓磊[1] 张丽[1] 

机构地区：[1]大连医科大学微生物教研室,大连116027

出　　处：《中国免疫学杂志》2003年第6期397-400,共4页Chinese Journal of Immunology

基　　金：辽宁省自然科学基金 (项目编号 :0 0 2 2 5 0 0 3 )

摘　　要：目的 :研究参杞合剂 (SQ)对小鼠腹水型肝癌细胞免疫功能及细胞周期的影响。方法 :用参杞合剂对荷瘤鼠进行连续胃饲治疗 10天 ,观察其抑瘤率及免疫学指标的改变。流式细胞仪结合电镜观察其细胞周期的改变。结果 :荷瘤小鼠抑瘤率为 6 5 6 8% ,SQ能增强荷瘤小鼠的免疫功能 (P <0 0 5 ) ;流式细胞仪检测肿瘤细胞形成S期阻滞。病理可见大片坏死 ,电镜可见坏死及凋亡。结论 :参杞合剂可显著抑制肿瘤生长 ,其机理与提高机体免疫功能 ,阻滞肿瘤细胞增殖有关。Objective:To study the inhibitive effect of SQ on HcaF 16 A 3 cells in vivo.Methods:SQ was given to BALB/C mice after bearng tumors for 10 consecutive days.The therapeutic effect was determined by measurement to tumor weight?immunological function and morphological changes.Flowcytometry and electronic microscope were used to evaluate the distribution of cell cycle.Results:The inhibition rate was 65.68%,NK/Mφ activities and cytokine production were enhanced by SQ,cell cycle was stopped at the S phase.Necrosis could be seen nuder microscope and characteristic apoptotic body could be seen under electronic microscope.Conclusion:SQ had a significant anti tumor effect by regulate immune system and inhibit tumor cell proliferation.

关 键 词：参杞合剂 荷瘤小鼠 免疫功能 细胞周期 影响 肿瘤免疫 

分 类 号：R285.5[医药卫生—中药学] R730.3[医药卫生—中医学]