新疆、辽宁地区HIV-1感染者细胞因子特征研究  

Study on character of cytokines of HIV-1 positive individuals in Xinjiang and Liao- ning

作　　者：张旻[1] 尚红[1] 张远志 周立平[1] 王亚男[1] 李丹[1] 张蕴秀[1] 孙峥嵘[1] 文小宁[1] 

机构地区：[1]中国医科大学附属第一医院艾滋病研究室,沈阳110001 [2]新疆维吾尔自治区疾病控制与预防中心

出　　处：《中国免疫学杂志》2003年第6期418-420,共3页Chinese Journal of Immunology

基　　金：<国家重大基础研究规划>973资助项目 (G19990 5 410 7) ;国家自然科学基金项目 ( 3 0 2 71188)

摘　　要：目的 :了解新疆、辽宁地区HIV 1感染者细胞因子特征及其与病程的关系。方法 :采用流式细胞仪测定CD4 + T淋巴细胞数 ,34例感染者按 1993年美国CDC艾滋病监测病例分类及扩大的诊断标准分A、B两组 ,ELISA方法检测血浆细胞因子IL 12、IFN γ、IL 10、IL 6水平 ,载量仪测定HIV 1感染者体内病毒载量 ,同时检测 16例健康对照者的血浆细胞因子。结果 :①与健康对照相比 ,HIV 1感染者血浆IL 12水平显著降低 (P <0 0 1) ,IFN γ、IL 10水平显著升高 (P <0 0 1) ,IL 6水平明显升高 (P <0 0 5 )。②A组血浆IFN γ水平明显高于B组 ,IFN γ水平降低与疾病进展密切相关 (P <0 0 5 )。结论 :①新疆、辽宁地区HIV 1感染者血浆细胞因子IL 12、IFN γ、IL 10、IL 6出现异常变化 ;②HIV 1感染者的IFN γ水平可辅助判断机体细胞免疫水平预测疾病进展。Objective:To investigate the character of cytokines and the relationship between cytokine level and disease progression of HIV 1 positive individuals in Xinjiang and Liaoning.Methods:34 HIV 1 infected patients and 16 normal controls were selected.CD4 + T lymphocytes were measured by flow cytometry.According to 1993 USA CDC AIDS surveillance case definition for adolescents and adults,34 HIV 1 infected patients were divided into two groups,A and B.The plasma levels of the cytokines IL 12,IFN γ,IL 10,IL 6 and viral load were measured by ELISA and RT PCR respectively.Also,the cytokine levels in plasma from 16 seronegative persons were measured.Results:①Comparing with the normal controls,the level of plasma IL 12 in the infected individuals was lower(P<0.01),the level of plasma IFN γ and IL 10 were higher(P<0.01),the level of plasma IL 6 was higher(P<0.05).②The plasma IFN γ level of group A was much higher than that of group B,and there was a positive correlation between the decreasing level of IFN γ and disease progression(P<0.05).Conclusion:①The abnormal changes of cytokines level of HIV 1 infected patients in Xinjiang and Liaoning appeared.②The level of plasma IFN γ may be used to evaluate the function of cellular immunity and to predict the progression of disease.

关 键 词：新疆 辽宁 HIV—1感染 细胞因子 诊断标准 

分 类 号：R512.91[医药卫生—内科学]