猪瘟病毒野毒株持续感染细胞模型的稳定性研究  

A Research of The Stability Based on A Cell Model Persistently Infected By A Field-isolated Virulent Strain of CSFV

作　　者：许振辉[1] 应松成[1] 沈超[1] 屈三甫[1] 郑从义[1] 王在时[2] 

机构地区：[1]武汉大学生命科学学院,湖北武汉430072 [2]中国兽药监察所,北京100081

出　　处：《中国病毒学》2003年第3期271-274,共4页Virologica Sinica

基　　金：国家重点基础研究发展规划项目"973"(G1999011904)

摘　　要：以本实验室建立的CSFV39-PK15持续感染细胞模型为实验材料,综合运用免疫荧光、RT-PCR、流式细胞仪,对其稳定性进行研究。实验结果均表明,该细胞模型有着良好的稳定性。即使在连续传至128代的CSFV39-PK15传代细胞中,CSFV仍持续存在:呈免疫荧光抗体反应阳性和RT-PCR检测阳性。同时,该细胞与正常的PK-15细胞相比,细胞周期无显著差异。通过同段序列的同源性比较,发现CSFV39与CSFV石门株的同源性最高,达99.02％。We jointly applied immunofluorescence technique, RT-PCR assay and flow cytometer to evaluate the stability of a cell model persistently infected by Classical swine fever virus (CSFV39), which was established by our laboratory and named CSFV39-PK15. The experiment results indicated that this cell model was quite stable. Even in the 128th CSFV39-PK15 passage cells, we still could detect the existence of CSFV39. Both immunofluorescence antibody test and RT-PCR assay showed positive results. We also found there was no significant difference in their cell cycle between normal control PK-15 and CSFV39-PK15. Sequence homology analysis of the same fragment revealed that CSFV39 had the highest homology compared with Shimen strain, amounting to 99.02%.

关 键 词：猪瘟病毒 野毒株 稳定性 细胞模型 持续感染 细胞周期 

分 类 号：S852.65[农业科学—基础兽医学]