对虾白斑综合症病毒与虾鳃细胞膜特异性结合关系的确立  

Verifying the Specific Binding Between WSSV and Shrimp Gill Cell Membrane

作　　者：易志刚[1] 黄倢[1] 解飞霞[2] 贾佩侨[1] 李筠[2] 

机构地区：[1]中国水产科学研究院黄海水产研究所,青岛266071 [2]中国海洋大学海洋生命学院,青岛266003

出　　处：《中国病毒学》2003年第3期295-297,共3页Virologica Sinica

基　　金：国家重点基础研究发展规划项目课题资助(G1999012002)

摘　　要：对虾白斑综合症病素养(White spot syndrome virus,WSSV)是养殖对虾的一个主要病原,也是目前发现的基因组最大的动物病素养(基因组约290kDa,双链环状).Cell membrane extracted from shrimp gill was coated on 96 well plate and bound with DIG labeled White spot syndrome virus (WSSV), then detected with Anti-DIG-Fab-HRP system, meanwhile taking the cell membrane from hepatopancreas, muscle, and E.coli for control group, the result verified the specific binding between WSSV and gill cell membrane. And here the binding assay can be employed as a convenient and rapid way to evaluate the binding nature between WSSV and the shrimp gill cell membrane.

关 键 词：对虾白斑综合症病毒 虾鳃 细胞膜 特异性结合 

分 类 号：S945[农业科学—水产养殖]