痕量雌二醇的吸附方波伏安法检测  

Adsorptive Square-wave Voltammetric Determination of Trace Estradiol in the Presence of Cetyltrimethylammonium Bromide

作　　者：何琼[1] 袁帅[2] 胡胜水[2] 

机构地区：[1]云南曲靖师范学院化学系,云南曲靖655000 [2]武汉大学化学与分子科学学院,武汉430072

出　　处：《分析科学学报》2003年第3期209-212,共4页Journal of Analytical Science

基　　金：国家自然科学基金 ( No.60 1 71 0 2 3 ) ;湖北省自然科学基金 ( No.99J0 60 ) ;云南曲靖师范学院科研项目 ( No.0 1 1 3 90 4)

摘　　要：本文报道一种简便、快速、灵敏检测痕量雌激素的吸附溶出方波伏安法。该法先将雌二醇在玻碳电极上吸附富集一定时间 ,然后以方波伏安溶出法检测其氧化电流。介质为含有 8× 1 0 - 5mol· L- 1溴化十六烷基三甲铵 ( CTAB)的 0 .1 mol· L- 1磷酸盐缓冲溶液 ( p H=1 0 .0 )。该法检出限为 5× 1 0 - 8mol· L- 1,线性范围为 5 .2× 1 0 - 7～ 5× 1 0 - 5mol· L- 1。A sensitive, simple and rapid adsorptive stripping procedure for the determination of trace estradiol has been developed by square wave voltammetry. The method was based on the adsorptive accumulation of estradiol on glassy carbon electrode in 0.1 mol·L -1 phosphate buffer solution (pH=10.0) by square wave stripping procedure. An oxidation peak was obtained. The peak current enhanced greatly in the presence of small amount of cationic surfactant cetyltrimethylammonium bromide (CTAB). A detection limit of 5×10 -8 mol·L -1 of estradiol was achieved. Experimental parameters were optimized for the determination of estradiol. A linear relationship between the peak current and the concentration of estradiol was established from 5.2×10 -7 mol·L -1 to 5×10 -5 mol·L -1 . The electrode process and adsorption mechanism of estradiol on the glassy carbon electrode were studied.

关 键 词：痕量分析 雌二醇 吸附方波伏安法 检测 电极反应机理 溴化十六烷基三甲铵 雌激素 电化学分析 

分 类 号：Q579.13[生物学—生物化学] Q503