四川大头茶在不同生境中的遗传分化  

GENETIC DIFFERENTIATION OF GORDORNIA ACUMINATA POPULATIONS IN DIFFERENT HABITATS

作　　者：操国兴[1] 谢德体[1] 钟章成[2] 刘芸[2] 

机构地区：[1]西南农业大学资源环境学院 [2]西南师范大学生命科学院,重庆400715

出　　处：《西南农业大学学报（自然科学版）》2003年第3期200-202,共3页Journal of Southwest Agricultural University

基　　金：国家自然科学基金重点盗助项目(39330050);四川省教育厅重点项目(2002 A 005)

摘　　要：采用随机扩增多态性DNA(RAPD)技术分析了缙云山四川大头茶(Gordornia acuminata)在3个群落类型(针阔混交林、四川大头茶纯林和常绿阔叶林)中的遗传多样性和遗传分化。12个引物共扩增出108个产物,其中82个是多态性的,多态位点比率为74％.Shannon指数估算的3个种群的平均遗传多样性为2.2448,总遗传多样性为2.6353,在总遗传变异中,有89.35％存在于种群内,10.65％存在于种群间。对上述研究结果进行了分析和讨论。RAPD (random amplified polymorphic DNA) technique was used to analyze genetic diversity and genetic differentiation of Gordonda acuminata populations in three different communities, i.e. G. acuminata coniferous mixed forest, G. acuminata pure forest and G. acuminata evergreen broad - leaved forest. Twelve random primers produced a total of 108 bands, of which 82 were polymorphic, with a polymorphic locus percentage of 73.6% . Estimated with Shannon index, the average genetic diversity of the populations was 2.2448 and their total genetic diversity was 2.6353. Intra-populational genetic diversity accounted for 89.53% of the total while inter-populations! genetic diversity accounted for 10.65% of the total.

关 键 词：RAPD 四川大头茶 种群 遗传分化 

分 类 号：S794.9[农业科学—林木遗传育种]