肾综合征出血热早期诊断与中药抗病毒的实验研究  

Experimental Studies on Early Diagnosis of HFRS and Viricidal Effects of Radix Isatidis

作　　者：李闻文[1] 阎祖炜[1] 龚道科[1] 戴敢[1] 刘水平[1] 

机构地区：[1]中南大学湘雅医学院检验系临床微生物学免疫学教研室,中国湖南长沙410078

出　　处：《实用预防医学》2003年第3期311-314,共4页Practical Preventive Medicine

摘　　要：目的　为肾综合征出血热病毒感染早期诊断和中药治疗提供实验依据。　方法　将肾综合征出血热病毒(HFRSV)接种于 Vero- E6细胞 ,培养后制成高效价病毒抗原片。应用酶免疫组化技术进行检测。采用中草药板兰根制剂体外作用病毒 ,比较不同浓度板兰根抗病毒的效果 ,培养后进行观察。　结果　酶免疫组化技术对流行性出血热能早期、快速、特异性地诊断。板兰根具有杀病毒作用。　结论　实验证明该法具有早期诊断、快速简便、特异敏感等优点 ,尤其适用于基层医疗单位推广应用。Objective To provide experimental evidence for clinical use of some anti-virus Chinese herbal medicine and the early diagnosis of hemorrhagic fever with renal syndrome virus(HFRSV). Methods HFRSV were inoculated with Vero-E6 cells. After culture, the high titer virus antigenic slides were made and applied to detect the serum anti-HFRSV antibody titer by inmuno-enzymatic histochemical assay.HFRSV infected cells were treated with Radix isatidis for 7 days, then we compared the anti-virus effect of different concentration. Result Using HFRSV antigenic slides by immuno-enzymatic histochemical assay to detect anti-HFRSV has high specificity and sensitivity. It is a rapid and simple method which can be used in HFRS infection diagnosis.It is superior in early diagnosis,specificity,sensitivity and simplicity.The result showed that 1/100 Radix isatidis had viricidal effect against HFRSV. Conclusion The virus antigenic slides can be used in HFRS diagnosis. The method is suitable for basic-level hospital.It provides an experimental evidence for clinical use of some Chinese herbal medicine.

关 键 词：肾综合征出血热病毒 免疫组化技术 诊断 中草药(板兰根) 

分 类 号：R512.8[医药卫生—内科学]