cAMP对胰岛素刺激的NIH3T3细胞丝裂原激活蛋白激酶活性的抑制作用  

Inhibition of cAMP on Mitogen-activated Protein Kinases Activity of NIH3T3 Cells Stimulated by Insulin

作　　者：何艳[1] 叶庆林[1] 林德馨[1] 高永琳[1] 

机构地区：[1]福建医科大学生物化学与分子生物学系,福州350004

出　　处：《福建医科大学学报》2003年第2期144-146,共3页Journal of Fujian Medical University

基　　金：福建省教育厅科研基金资助项目 ( JA99183 )

摘　　要：目的　探讨 c AMP- PKA信号途径对胰岛素激活的 Ras-丝裂原激活蛋白激酶 (MAPK)信号途径的影响。　方法　以 3-异丁基 - 1-甲基黄嘌呤 (IBMX)提高细胞内环腺苷酸 (c AMP)浓度 ,藉四唑蓝比色法 (MTT法 )和蛋白免疫印迹试验分别观察 c AMP对胰岛素刺激的小鼠成纤维细胞 (NIH3T3细胞 )的生长增殖和胞内丝裂原激活蛋白激酶(MAPK)活性的影响。　结果　 IBMX(0 .5 mmol/ L )抑制静息的和胰岛素 (10 m U / m L )刺激的 NIH3T3细胞的增殖 ,作用呈时间依赖性。在胰岛素 (10 m U/ m L)刺激的 NIH3T3细胞 ,MAPK出现酪氨酸磷酸化 ;而以 IBMX(0 .5 mmol/L)预处理细胞 30 min后 ,再以胰岛素作用 10 min,胰岛素刺激的 MAPK的酪氨酸磷酸化受抑制。　结论　 c AMP可通过抑制 MAPK磷酸化活化对Objective To study the effect of cAMP\|PKA signal transduction pathway on Ras\|\{mitogen\}\|activated protein kinases(MAPK) signal pathway stimulated by insulin in NIH3T3 cell. \{Methods\} The effect of 3\|isobutyl\|1\|methylxenthine(IBMX) which increases the intracellular cAMP on the growth and activity of MAPK of NIH3T3 cells stimulated by insulin were investigated with methylthiazolyl tetrazolium(MTT) assay and Western\|blot respectively. Results IBMX(0 5 mmol/L) inhibited the proliferation of NIH3T3 cells with or without 10 mU/mL insulin. Tyrosine phosphorylation of MAPK in NIH3T3 cells stimulated with insulin was decreased when it was pretreated with IBMX for \{30 min\}. \{Conclusion\} cAMP may inhibit the growth of NIH3T3 cells by inhibiting the Ras\|MAPK signal pathway.

关 键 词：信号传导 胰岛素 环AMP 有丝分裂素激活蛋白激酶类 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]