应用浸种法导入外源DNA转化烤烟变异株D_2代的氨基酸含量分析  

Analysis Of Amino Acids Content From the Genetic Mutation of D_2 Generation Tobacco Transformed with Foreign DNA by Seed-Immersion Method

作　　者：杨丽娟[1] 白岩[2] 白嵩[3] 

机构地区：[1]东北农业大学生命科学学院 [2]吉林省生物研究所 [3]吉林农业大学

出　　处：《农业与技术》2003年第3期69-72,共4页Agriculture and Technology

摘　　要：本文对应用浸种法将烤烟CV87总DNA导入受体烤烟NC89产生的抗赤星病变异株D2代的水 解氨基酸含量进行了分析。结果表明,变异株D2代4个抗病株系的17种氨基酸总量均比受体低。通过 与受体和供体各种氨基酸成分的相对含量比较表明,这4个变异株系中的天冬氨酸、丙氨酸、缬氨酸、 甲硫氨酸、异亮氨酸、亮氨酸、酪氨酸、苯丙氨酸、赖氨酸、精氨酸、苏氨酸、丝氨酸、谷氨酸、甘氨 酸、胱氨酸含量的改变与供体烤烟CV87的DNA导入有关。We analyzed hydrolysis amino acids content of resistant red star disease from mutant D2 generation that tobacco CV87 was transformed into Tobacco NC89 by Seed-Immersion Method. The result indicated that the content of all 17 amino acids of the mutant D2 4 plants that resistant to red star disease was lower than those receptors.The comparison of amino acid relative content between the donar and receptor showed that the transformation of the donar tobacco CV87 DNA was the reason that the change of amino acids content: Asp、Ala、Arg、Val、Met、Ile、Leu、Tyr、Phe、Lys、Thr、Ser、Glu、Gly and Cys.

关 键 词：烤烟 外源DNA 转基因 变异株 氨基酸含量 浸种法 赤星病 抗病性 

分 类 号：S572[农业科学—烟草工业] S336[农业科学—作物学]