一种双水相亲和分配配基的制备过程优化——亚氨基二乙酸-聚乙二醇  

Synthesis Process of an Affinity Ligand for Aqueous Two-phase Partitioning-Iminodiacetic Acid-Polyethylene Glycol

作　　者：文禹撷[1] 林东强[1] 陆瑾[1] 姚善泾[1] 

机构地区：[1]浙江大学化学工程与生物工程系,浙江杭州310027

出　　处：《化学反应工程与工艺》2003年第2期129-134,共6页Chemical Reaction Engineering and Technology

基　　金：国家自然科学基金资助项目(编号:20076042)

摘　　要：　考察了不同亚氨基二乙酸(IDA)取代度的亚氨基二乙酸-聚乙二醇(IDA-PEG)的制备,并进行了条件优化(BF3浓度,NaOH浓度,反应时间,IDA结合条件等)。通过控制环氧氯丙烷与聚乙二醇的摩尔配比,得到了不同Cu( )含量的固定化金属亲和配基:Cu( )-IDA-PEG(A)(0.24molCu2+/molPEG)、Cu( )-IDA-PEG(B)(0.51molCu2+/molPEG)、Cu( )-IDA-PEG(C)(0.75molCu2+/molPEG),并初步探讨了固定化金属亲和配基的添加对PEG4000-(NH4)2SO4-H2O双水相系统相图的影响。The synthesis process of iminodiacetic acid  polyethylene glycol (IDAPEG) with varying content of IDA was investigated. The process was optimized under BF3 concentration, NaOH concentration, reaction time, and IDA coupling conditions. It was found that the amount of copper (Ⅱ) on the immobilized metal ion affinity ligand is related to the molecular ratio of epichlorohydrin to PEG in the synthesis process. Under optimal conditions, three affinity ligands of Cu(Ⅱ)-IDA-PEG were prepared with different Cu(Ⅱ) concentrations, 0.24 mol/molPEG, 0.51 mol/molPEG, 0.75 mol/molPEG, respectively. The effect of the immobilized metal ion affinity ligand on the phase diagram of PEG4000(NH4)2SO4H2O aqueous twophase systems was discussed.

关 键 词：双水相亲和分配配基 制备过程 优化 亚氨基二乙酸 聚乙二醇 固定化金属离子亲和层析 分离 蛋白质 

分 类 号：Q51[生物学—生物化学] Q503