烟台地区肾综合征出血热患者血清抗体检测和汉坦病毒序列测定  

Serum antibody response and Hantavirus RNA sequencing in patients with hemorrhagic fever renal syndrome in Yantai areas

作　　者：范淑珍 牟广臻 宫连凤 石义全 高少波 邵长平 裴贵忠 王传良 孙颖 

机构地区：[1]山东烟台市卫生防疫站防疫科,烟台264001

出　　处：《中华实验和临床病毒学杂志》2003年第2期124-128,共5页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　了解烟台地区肾综合征出血热 (HFRS)患者血清中IgG、IgM抗体水平 ,确定引起该地区HFRS流行的汉坦病毒的型别分布。方法　收集临床HFRS急性期和恢复期患者血清 ;用ELISA检测IgG、IgM抗体 ;用交叉空斑减少中和试验检测中和抗体 ;采用Trizol法提取患者血清中HFRS病毒RNA ,用套式PCR产物做TA试验 ,测定核苷酸序列。结果　HFRS患者血清IgM阳性率为82 2 % (88 10 7) ,IgG阳性率为 85 7% (6 6 77)。该地区两城市 38份HFRS患者血清中 ,有 32份属家鼠型病毒 (SEO)感染 ,另 6份未能定型 ;另一城市 16份HFRS患者血清中 ,有 15份属姬鼠型病毒感染 ,1份未定型。该地区HFRS病毒与SEO的同源性达 90 %以上。结论　引起烟台地区HFRS流行的汉坦病毒 ,属于以SEO为主的混合型病毒。Objective To understand antibody responses to and RNA sequences of Hantavirus in patients with hemorrhagic fever renal syndrome (HFRS) in Yantai areas and to demonstrate the type of the prevalent viruses caused HFRS Methods Serum specimens collected at acute and convalescent stages from 90 patients with HFRS and IgM and IgG antibodies against Hantavirus were detected with ELISA, and cross plaque reduction neutralizing tests were performed to detect neutralizing antibody Viral RNA was extracted from the patients′sera by using Trizol method and nested PCR was utilized to amplify the specific segments of the viral cDNA and the products of the PCR were TA cloned and then the nucleotide sequences were determined Results The IgM antibody was positive in 82 2% (88/107) of the patients while the IgG antibody was positive in 85 7% (66/77) of the patients Both the serologic and sequence analyses demonstrated that the epidemic of HFRS in Yantai areas was caused by mixed types of Hantavirus The prevalent strains of Hantavirus had higher homology with the strains isolated in Korea than with those isolated previously in China Conclusion The serologic and sequencing analyses indicated that the epidemic of HFRS in Yantai areas was caused by mixed types of Hantavirus dominated by type SEO

关 键 词：烟台地区 肾综合征出血热 血清 抗体 检测 汉坦病毒 测定 基因 核苷酸序列 

分 类 号：R512.8[医药卫生—内科学]