慢性肝炎患者ECR1基因多态性、数量及活性的变化  

Changes of ECR1 genomic density polymorphism, quantitative expression and the activity of ECR1 natural adhesion in patients with chronic hepatitis

作　　者：毛远丽[1] 王海滨[1] 孙志强[1] 崔恩博[1] 马洪滨[1] 鞠连才[1] 姜平[1] 

机构地区：[1]解放军第三○二医院临床检验中心,北京100039

出　　处：《中华实验和临床病毒学杂志》2003年第2期146-148,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　研究慢性肝炎 (CH)患者红细胞Ⅰ型补体受体 (ECR1)密度相关基因多态性、数量表达及活性的变化。方法　采用PCR和HindⅢ酶切技术测定ECR1分子基因多态性 ,采用酶联免疫法定量测定ECR1分子的数量 ,采用红细胞天然免疫粘附功能试验测定ECR1粘附活性。结果　慢性肝炎患者ECR1密度相关基因多态性与健康人群相比差异无显著性。活动性慢性肝炎、肝功能异常的肝炎后肝硬化患者ECR1的数量表达及活性明显低于健康人群 (t=9 87,P <0 0 0 0 1) ,失代偿性肝炎后肝硬化患者的ECR1分子数量明显低于代偿性肝炎后肝硬化患者 ,但肝功能正常的慢性肝炎患者ECR1数量与健康人群比较无明显变化。结论　慢性肝炎患者ECR1数量表达及活性缺陷系后天引起 ,测定慢性肝炎患者ECR1的数量对临床病情判断及发展预测有重要参考价值。Objective To study the changes of genomic density polymorphism, quantitative expression and the adhesion activity of complement receptor type 1 (ECR1) on erythrocytes in patients with chronic hepatitis Methods Polymerase chain reaction (PCR) and Hind Ⅲ restriction enzyme digestion, the quantitative assay of ECR1 and the activity of erythrocytes immune adhesion test were applied Results The spot mutation rate (25 0% 30 3%) of ECR1 density gene in patients with chronic hepatitis was not significantly different from that of healthy individuals (28 0%) The amount of ECR1 in patients with chronic hepatitis, except for the diseases with normal liver function, was significantly lower than that of healthy individuals ( t =9 87, P <0 000 1) The quantitative expression of ECR1 in decompensated cirrhosis was obviously lower than that of compensated cirrhosis( t =2 21, P <0 05) Conclusion Defective expression of ECR1 in chronic hepatitis B may be acquired through central and/or peripheral mechanisms It is very important to study the quantitative expression in the patients with chronic hepatitis

关 键 词：慢性病毒性肝炎 ECRl 基因多态性 红细胞I型补体受体 粘附活性 数量表达 

分 类 号：R512.6[医药卫生—内科学]