热毒清眼药水体外抗Ⅰ型单纯疱疹病毒药效的评价  

Eveluation on reducing effect of eye drop on herpes simplex virus-Ⅰ in vitro

作　　者：钟良玉[1] 陈静宜[2] 陈红梅[2] 徐玮[2] 王晓萌[1] 来坚[1] 柳雅琴[1] 

机构地区：[1]杭州市中医院眼科,310007 [2]杭州市中医院制剂室,310007

出　　处：《中华实验和临床病毒学杂志》2003年第2期183-186,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：浙江省中医药科研基金 ( 980 3 9)

摘　　要：目的　通过组织细胞培养实验观察中药热毒清眼药水 (RED)对单纯疱疹性病毒 (HSV)所致细胞病变影响。方法　最大无毒浓度的热毒清眼药水分别对 10 0TCID50 与 10TCID50 单纯疱疹病毒Ⅰ型感染细胞所致的细胞病变进行用药抑制和检测热毒清眼药水对单纯疱疹病毒 Ⅰ (HSV Ⅰ )病毒经不同浓度药物处理后所致细胞病变的影响。结果　最大无毒浓度的热毒清眼药水对 10 0TCID50 与10TCID50 HSV Ⅰ致细胞病变有明显抑制作用 ,与 0 1%阿昔洛韦眼药水抑制作用比差异无显著性(P >0 0 5 ) ,两者均未出现细胞病变 ,而与对照组比差异有显著性 (P <0 0 5 )。 5 0 0 0 0 0～ 6 30 0 0 μg L热毒清眼药水对HSV Ⅰ致细胞病变抑制作用比 2 0 0 0 0 0 0 μg L～ 10 0 0 0 0 0 μg L两种浓度更强 ,与对照组比差异有非常显著意义 (P <0 0 1)。热毒清眼药水细胞最大耐受浓度是 2 0 0 0 0 0 0 μg L(1∶80稀释度 ) ,大于阿昔洛韦眼药水细胞最大耐受浓度 5 0 0 0 μg L(1∶4 0 0稀释度 )。 结论　实验证实热毒清眼药水具有明显抗HSV Ⅰ作用。热毒清眼药水细胞毒性很小 ,明显低于阿昔洛韦眼药水。Objective To observe the effect of herbal eye drop Reduqing (clearing toxic heat) on herpes simplex virus (HSV) on the cytopathic effect in cell culture in vitro Methods The maximal non toxic Reduqing eye drop concentration added to cell culture infected with 100 and 10TCID 50 of herpes simplex virus Ⅰ (HSV Ⅰ) to observe the inhibitory effect of the medicine on HSV Ⅰ induced cytopathic effect Results The Reduqing eye drop at maximal non toxic concentration could abviously inhibit the cytopatic effect induced by 100TCID 50 and 10TCID 50 HSV Ⅰ No statistically significant difference was found when compared with 0 1% acyclovir eye drop( P >0 05), on cytopathic effect occurred with boty eye drops However, there was significant difference when compared with control group( P <0 05) The inhibitory effect of concentrations of Reduqing at 500 000 and 63 000 μg/L was stronger than that of 2 000 000 and 1 000 000 μg/L ( P <0 01) The maximal tolerabte concentration of Reduqing eye drop by the cell was 2 000 000 μg/L (1∶80 dilution), which was higher than that of 5 000 μg/L(1∶400 dilution) acyclovir eye drop Conclusions These re sults wuggest that Reduqing eye drop could markedly inhibit the cytopathic effects caused by HSV Ⅰ The Reduqing eye drop showed remarkably low toxic effct to the cells as compared to acyclovir eye drop

关 键 词：热毒清眼药水 I型单纯疱疹病毒 药效学 中药 抗病毒 单纯疱疹性角膜炎 实验 

分 类 号：R276.7[医药卫生—中医五官科学]