嗜凤梨果蝇(Drosophila ananassae)细胞系的建立及其生物学特性初步观察  

Establishment of Drosophila ananassae cell line and its biological characteristics

作　　者：洪鹰[1] 曾庆韬[1] 

机构地区：[1]湖北大学生命科学学院,湖北武汉430062

出　　处：《湖北大学学报（自然科学版）》2003年第2期144-147,共4页Journal of Hubei University：Natural Science

基　　金：国家自然科学基金重点项目(39930100)

摘　　要：为建立真核基因表达受体系统,以嗜凤梨果蝇胚胎为材料,采用常规方法获取发育4～8h的果蝇胚胎细胞,以改良M3(BF)培养液体外培养,经40d左右的原代培养后行传代培养,以后每隔7d传代一次,传至10代时按照细胞系建立标准检测细胞系的一系列生物学特性.结果显示:细胞维持体外生长将近1年,传至60代.细胞在接种的0～96h内呈指数生长,临界增殖浓度为1.0×106个(细胞) mL.部分细胞染色体呈现异倍化.经液氮冻存的细胞复苏后活性达90%以上.该细胞系是一株成功的细胞系,命名为HY-ANA.In order to establish eukaryotic gene expression system, embryos of Drosophila ananassae were used as experimental materials.Broked the embryos that had developed for 4～8 hours into cells by using conventional method, after that cultured the cells in improved M3(BF) medium in vitro. As for 40 days primary culture the cells could be passaged one time every 7 days . During this time some cells were frozen in liquid nitrogen. When the cells were passaged to 10 generation-times a series of characteristics of the cell line was examined according to the criterion of a successful cell line. Conclusion: cells were maintained in good living state for about one year, passaging to 60 generation-times. Cells proliferated at exponent from 0 hour to 96 hours after plating. The lowest concentration in which cells could proliferate was 1.0×106/mL. Parts of cells became heteroploid. The viability of the cells recovered from liquid nitrogen was up to 90% or above. The cell line was a successful cell line,named HY-ANA.

关 键 词：嗜凤梨果蝇 细胞系 生物学特性 真核基因表达受体系统 细胞培养 原代培养 传代培养 

分 类 号：Q969.453.8[生物学—昆虫学] Q813.11