“参芪脑保”治疗大鼠局灶脑缺血及再灌注损伤实验研究  

The effect of Senqi naobao on reperfusion injury after focal cerebral ischemia in rat model with thread embolism method

作　　者：杜卫[1] 尹岭[1] 金香兰[1] 武晓玲[2] 宋春杰[1] 孙锦平[1] 朱克[1] 

机构地区：[1]北京解放军总医院神经信息中心,100853 [2]解放军第251医院

出　　处：《卒中与神经疾病》2003年第3期147-150,共4页Stroke and Nervous Diseases

基　　金：九五全军医药卫生科研基金重点课题基金资助 (编号 :95Z0 5 8)

摘　　要：目的　研究中药“参芪脑保”对脑缺血 再灌注损伤的治疗作用。方法　用线栓法制作大鼠大脑中动脉缺血 6小时再灌注模型 ,将大鼠随机分为对照组 (2 0只 )和参芪脑保治疗组 (2 0只 )。治疗组于术后 3小时始连续 7天给予参芪脑保灌胃治疗 ,观察神经功能缺损评分变化。各组大鼠于用药 7天后处死 ,行TTC和HE染色 ,测定脑梗死体积及神经元损伤情况。结果　参芪脑保治疗组大鼠 4 8小时死亡数 5只 (占2 5 % )低于对照组 10只 (占 5 0 % ) ,而 7天存活数 15只 (占 75 % )高于对照组 10只 (占 5 0 % )。参芪脑保治疗组梗死灶体积 (2 6 .5± 5 .2 )mm3 明显小于对照组 (4 1.9± 7.3)mm3 (P <0 .0 0 1) ,神经元缺失、脑梗死灶内出血较对照组减小。结论　“参芪脑保”对脑缺血 再灌注损伤有保护作用。Objective To study the effect of Senqi Naobao on reperfusioninjury after focal cerebral ischemia.Methods The model of focal cerebral ischemia for 6 hours and reperfusion was made by thread embolism method.Rats were randomly divided into two groups: control group (n=20) and Senqi naobao treatment group (n=20).In treatment group, 3 hours after operation, Senqi Naobao was poured into stomach continuously for 7 days and changes of neurological scores in rats were observed.All rats were killed at the 7th day after treatment.Infarction size was determined in triphenyltetrazolium chloride stained sections and ischemic lesions of neurons were examined microscopically in HE stained sections.Results Number of death in treatment group was lower significantly than control group, and the number of survival rats in treatment group was higher than control group.In Senqi Naobao treatment group, infarction size was reduced significantly than control group (P< 0.001), and neuron depletion and hemorrhage in infarction size were lower.Conclusions Senqi Naobao is effective in the treatment of reperfusion injury after focal cerebral ischemia.

关 键 词：参芪脑保 中医药治疗 大鼠 局灶脑缺血 再灌注损伤 实验 

分 类 号：R277.733.1[医药卫生—中医学] R285.5