芪苓柴虎汤提取物抗乙型肝炎病毒的细胞实验研究  

In-vitro Anti-HBV Effect of Extractive of Qilingcaihu Decoction

作　　者：金文君[1] 叶晓光[1] 唐正运[1] 黄子夏[1] 张楚营[1] 李扬彬[1] 刘国樵[2] 郭端莱[2] 

机构地区：[1]广州医学院第二附属医院,广州510260 [2]广州市医药工业研究所,广州510240

出　　处：《广州中医药大学学报》2003年第2期161-162,共2页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省中医药管理局2002年立项资助(编号:102050);广州市卫生局1999年立项资助项目[穗卫中(2000)1号]

摘　　要：[目的]探讨芪苓柴虎汤(OD)提取物的抗乙型肝炎病毒(HBV)作用。[方法]OD提取物处理乙肝病毒转染的细胞(2.2.15细胞),用ELISA法测定HBsAg和HBeAg。[结果]OD制成的4个不同工艺提取物对HBV-DNA转染的2.2.15细胞分泌的HBsAg、HBeAg均有明显的抑制作用,并呈一定的剂量依赖关系。[结论]芪苓柴虎汤在体外对HBV有一定的抑制作用,为临床治疗慢性乙型肝炎提供了实验依据。[Objective] To investigate in-vitro anti-HBV effect of extractive of Qilingcaihu Decoction (QD) . [Methods] HBsAg and HBeAg levels in the 2.2.15 cells treated with extractive of QD were examined by enzyme-linked immunosorbent assay. [Results] QD extractives processed by four methods inhibited the secretion of HBsAg and HBeAg in HBV-DNA-bearing 2.2.15 cells in a dose-effect manner. [Conclusion] QD has a certain inhibitory effect on HBV and this supply an experimental envidence for the treatment of chronic hepatitis B.

关 键 词：芪苓柴虎汤提取物 乙型肝炎 药理学 细胞学实验 抗病毒 

分 类 号：R259.126.2[医药卫生—中西医结合] R285.5[医药卫生—中医内科学]