反相高效液相法测定通痹灵片中马钱子碱、士的宁的含量  

Content Determination of Brucine and Strychnine in Tongbiling Tablet by Reversed-phase HPLC

作　　者：黄可儿[1] 柯雪红[1] 陈纪藩[1] 

机构地区：[1]广州中医药大学第一附属医院,广州510405

出　　处：《广州中医药大学学报》2003年第2期163-165,共3页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金(编号:39930230)

摘　　要：[目的]建立测定通痹灵片中马钱子碱、士的宁含量的方法。[方法]采用反相高效液相色谱法,色谱柱为NUCLEODUR C_(18) Gravity分析柱(125mm×4.6mm,5μm),流动相为甲醇—水(60:40)1000 mL中加入磷酸二氢钾0.5g、十二烷基磺酸钠1.0g、三乙胺0.5ml(冰醋酸调pH至4.5),检测波长254nm,流速1.0mL/min,室温操作。[结果]马钱子碱、士的宁平均回收率分别为97.69％(RSD=2.03％),97.93％(RSD=2.61％)。[结论]本方法快速准确,可作为通痹灵片的质控指标。[Objective] To establish a method for the determination of brucine and strychnine contents in Tongbiling Tablet. [Methods] Reversed-phase HPLC conditions were: Nucleodur C18 Gravity column (125 mm × 4.6 mm, 5μm), mobile phrase being methanol-water (60:40) with 0.5 g potassium dihydrogen phosphate and 1.0 g dodecyl sodium sulfouate and 0.5 mL triethylamine in 1 000 mL mixture (adjusting pH to 4.5 with acetic acid) , detecting wavelength at 254 run and a flow rate of 1.0 mL/min at room temperature. [ Results ] The average recovery of brucine was 97.69 % ( RSD = 2.03%) and that of strychnine 97.93% ( RSD = 2.61%). [Conclusion] This method is efficient and can be used for the quality control of Tongbiling Tablet.

关 键 词：通痹灵片 马钱子碱 士的宁 含量测定 反相高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学]