番茄外植体诱导直接分化不定芽建立高频再生系统  

Establishment of Tomato High-Frequency Regeneration System by Iduced Directly to Differentiate Buds

作　　者：李铁松[1] 王关林[1] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116029

出　　处：《辽宁师范大学学报（自然科学版）》2003年第2期178-182,共5页Journal of Liaoning Normal University:Natural Science Edition

摘　　要：研究以番茄(LycoperciconesculentumMill)栽培品种贵妃和碧娇为试材,通过对番茄子叶、下胚轴、叶片和茎段进行离体培养,不经愈伤组织阶段,诱导直接分化不定芽,研究并得到其高频再生的最适培养基和试管苗扦插繁殖的最适培养基,并对影响高频再生的其他因素做了研究,建立了再生频率高达98%以上的番茄高频再生系统,为番茄快速繁殖及基因转化研究奠定了基础.Tomato(Lycopercicon esculentum Mill) Cultivars,Guifei and Bijiao,were used as the materials in the study. The cotyledon, hypocotyl, leaf and stem of tomato were cultured in vitro and induced directly to differentiate buds.The optimal medium of highfrequency regeneration was investigated and obtained.The optimal medium of cutting breeding of tomato testtube seedlings was also obtained, the other factors affecting the highfrequency regeneration were also studied, and the highfrequency regeneration system of tomato was established with the regeneration frequency being above 98%, which became the important base of the highfrequency transformation of tomato.

关 键 词：番茄 直接分化 不定芽 高频再生系统 组织培养 基因转化 培养基 快速繁殖 

分 类 号：S641.203.6[农业科学—蔬菜学] S336[农业科学—园艺学]