反相高效液相色谱法制备桃叶珊瑚苷标准品  

Separation of Aucubin by Reversed-Phase Preparative High Performance Liquid Chromatography

作　　者：刘贤旺[1] 吴祥松[1] 黄慧莲[1] 赖学文[1] 黄桂林[1] 徐坚[1] 曹岚[1] 周至明[1] 

机构地区：[1]江西中医学院,南昌330006

出　　处：《江西中医学院学报》2003年第2期43-44,共2页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：国家自然科学基金资助课题(No.3 9960 0 82 )

摘　　要：利用反相高效液相色谱结合大孔吸附树脂柱色谱和硅胶干柱色谱法 ,从车前草的 70 %甲醇提取物中纯化制备了环烯醚萜苷类化合物———桃叶珊瑚苷的标准品 ,纯度达到 98%以上。该方法为桃叶珊瑚苷及其他环烯醚萜苷类化合物的制备提供了参考。Aucubin,an iridoid glucoside, was isolated from plantago herb by using sonication,AB 8 polymer adsorption column separation,classical silica gel column separation.Aucubin was finally purified by C 18 reversed phase preparative high performance liquid chromatography and was identified by thin layer chromatography.The purity of the product was over 98%.The chemical structure of aucubin was determined by 1 HNMR and 13 CNMR.\;In this work,the conditions of the reversed phase preparative HPLC technique to purify aucubin was optimized.The reversed phase preparative HPLC on a C 18 column with a mobile phase of methanol water(5∶95,v/v) and the technique of center cutting proved effective.\;

关 键 词：反相高效液相色谱法 制备 桃叶珊瑚苷 环烯酸醚萜苷 大孔吸附树脂柱色谱法 硅胶干柱色谱法 

分 类 号：R284.1[医药卫生—中药学]