含CpG免疫刺激序列的乙型肝炎DNA疫苗的体液免疫效果  

Humoral immune response induced by HBV DNA vaccine containing CpG ISS

作　　者：丘力功[1] 陈光明 张金华[1] 黄明 

机构地区：[1]广州市医药工业研究所,广东广州510240 [2]空军广州医院肝病研究所,广东广州510000 [3]广州药业集团,广东广州510000

出　　处：《中国生化药物杂志》2003年第3期118-120,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的构建含CpG免疫刺激序列的乙型肝炎DNA疫苗 ,评价其体液免疫效果。方法通过PCR方法从重组真核表达质粒pcDNA S2 S扩增出含CpG免疫刺激序列的乙型肝炎病毒 (HBV)表面抗原中蛋白 (preS2 +S)基因片段 ,将其亚克隆于含卡那霉素抗性基因的pVAX1真核质粒表达载体中 ,构建出重组真核表达质粒pVAX S2 S ,并按不同剂量一次性肌肉注射免疫BALB c小鼠。结果pVAX S2 S高、中、低 3个剂量组均能在 2周诱导抗 HBs产生 ,抗体效价随时间延长而增加。血清抗体水平比较 ,高剂量组 [(131.2 7± 2 5 .4 9)mIU ml]较中剂量组 [(5 6 .17± 12 .0 6 )mIU ml]、低剂量组 [(2 1.38± 7.73)mIU ml]高 ,差异均具非常显著性 (P <0 .0 1) ,以后第 4周高、中剂量组间差异依然非常显著 (P <0 .0 1) ,在第 8周高、中剂量间差别缩小 ,但两者较低剂量组差异均具非常显著性 (P <0 .0 1)。结论pVAX S2PurposeTo construct HBV DNA vaccine and to evaluate its humoral immune effect.MethodsThrough PCR, the HBV antigen middle protein gene containing CpG ISS was amplified from the recombinant eukaryotic expresssion plasmid pcDNA S 2S,then subcloned into eukaryotic expression vector pVAX1,so the recombinant eukaryotic expression plasmid pVAX S 2S was constructed and this recombinant plasmid was applicated to vaccinate the healthy BALB/c mice.ResultsSerum anti HBs appeared at the 2 nd week after DNA vaccination,and quantitative comparison of the serum Ab level revealed very significant( P <0.01)difference between the high dose group (131.27±25.49) mIU/ml and the median dose group(56.17±12.06 ) mIU/ml or the low dose group(21.38±7.73 ) mIU /ml. During the 8th week following ,the difference betweeen the high and median dose group became less remarkable whereas it remained very significant between the high and low dose group.ConclusionThe recombinant plasmid pVAX S 2S was effective to induce humoral immune response in healthy mice.

关 键 词：DNA疫苗 CpG免疫刺激序列 乙型肝炎表面抗原 体液免疫 

分 类 号：R392－33[医药卫生—免疫学] R978[医药卫生—基础医学]