利用激光扫描共聚焦显微镜的肿瘤血管在体成像研究  

The Study of Tumor Blood Vessel in vivo Imaging by Laser Scanning Confocal Microscope

作　　者：唐永红[1] 邢达[1] 高双荣[2] 钟雪云[2] 

机构地区：[1]华南师范大学激光生命科学研究所,中国广东广州510631 [2]暨南大学医学院病理教研室,中国广东广州510632

出　　处：《激光生物学报》2003年第3期184-186,194,共4页Acta Laser Biology Sinica

基　　金：国家重大基础研究前期专项(2002CCC00400);广东省自然科学基金团队项目(015012)资助。

摘　　要：传统的观察血管的方法需将组织制成切片,然后通过光学显微镜进行观察。显示的只是血管的某一片段,而无法观察到血管的全貌。应用激光扫描共聚焦显微镜,可对活体动物血管进行断层成像,从而再现血管的结构。本方法为对肿瘤等病变组织血管进行研究提供了一种新的检测手段。In traditional method of observing blood vessels, the samples must be made to be sections and then observed with microscope, in which only fragments of the blood vessels are observed and the full view of the blood vessels cannot. In this paper, we demonstrated a new method to study the morphology and structure of the blood vessels of tumor by laser scanning confocal microscope (LSCM). With the LSCM, we successfully realized high quality in vivo imaging of tumor blood vessels. 

关 键 词：激光扫描共聚焦显微镜 肿瘤 血管 荧光图像 

分 类 号：O439[机械工程—光学工程] R73[理学—光学]