用于改善聚醚酯细胞亲和性的两种表面改性方法  

Two methods of surface modification for Poly (ethylene oxide terephtalate)/Poly (butylene terephtalate) to improve its cell affinity

作　　者：白云[1] 朱吉力 杨新林[1] 孙涛[2] 王松灵[2] 

机构地区：[1]北京理工大学生命科学与技术学院生物技术系 [2]首都医科大学附属北京口腔医院涎腺疾病中心,北京市100050

出　　处：《中国临床康复》2003年第14期2024-2026,T002,共4页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金(30170254);北京市科技项目(H010910010113)~~

摘　　要：目的提高材料的细胞亲和性是生物材料改性研究的主要目的之一,采用两种表面处理方法对聚醚酯膜进行表面改性,比较两种改性方法对人涎腺细胞系(HSG)在材料上亲和性的影响。方法材料的表面性质通过接触角测量、X射线光电子能谱(XPS)进行表征。细胞形态和生长通过倒置显微镜观察、贴壁率测定和MTT测试来评价。结果两种方法处理后聚醚酯材料的水接触角都会降低,在用材料表面捕获明胶改性后,水接触角变化不大,从约82°下降到约71°,说明亲水性改变不大;而在用氨等离子体改性后材料的接触角显著降低,低于10°,说明材料的亲水性显著提高。细胞在培养6h后没有改性材料的细胞贴壁率很低,为21.2%,但是捕获和等离子体处理后,细胞的贴壁率分别上升到36.7%和67.4%,显示材料的细胞亲和性得到有效的提高。细胞在处理前后材料上培养72h的MTT测试结果:改性后材料上活细胞的数量要明显高于没有改性材料,细胞数量存在显著性差异,也说明改性后材料的细胞亲和性得到提高。在两种表面改性方法中,等离子体处理对改善材料的细胞亲和性更加有效。结论用捕获明胶和氨等离子体处理可以有效提高聚醚酯材料的细胞亲和性,尤其以直接等离子体处理更为有效。Aim To study the effects of two methods of surface treatment for Poly(ethylene oxide terephtalate)/Poly(butylene terephtalate)(PEOT/PBT) on the cell affinity of human salivary gland cells as the basis of the research on biomaterial modification.Methods Surface properties of modified materials were characterized by contact angle measurement,and X ay photoelectron spectroscopy(XPS).Cell morphological observation was carried out under invert microscopy.Cell attaching efficiency and MTT test were also used to evaluate the cell affinity of materials.Results Water contact angle decreased from 82°to 71°approximately after gelatin was successfully introduced into the surface of materials, with an insignificant rise in the hydrophilicity of the material. The anchoring rate of the cells cultured for 6 hours was 21.2%, significantly lower than those of the cells treated with gelatin(36.7%) and ammonia plasma(67.4%).It was indicated that the affinity of cells significantly rose after surface treatment.The MTT test showed that the number of reactive cells before treatment was less than that after treatment on the surface of the material cultured for 72 hours, with a significant difference.The plasma was more efficient in improvement of the cell affinity.Conclusions Surface treatment of gelatin and ammonia plasma treatment efficiently improve the cell affinity of PEOT/PBT.

关 键 词：聚醚酯 细胞亲和性 生物材料 表面改性 生物相容性 涎腺 等离子体 

分 类 号：R318.08[医药卫生—生物医学工程]