毛细管电泳法分离测定芦丁、槲皮素和连翘苷  

Separation and determination of rutin, quercetin and forsythin in the traditional Chinese herbs by capillary electrophoresis

作　　者：李向军[1] 张裕平[1] 袁倬斌[1] 

机构地区：[1]中国科学院研究生院化学部,北京100039

出　　处：《分析试验室》2003年第2期39-41,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金 (2 9875 0 2 7);国家自然科学基金分析化学重点基金 (2 0 2 35 0 10 )资助课题

摘　　要：用毛细管电泳紫外检测法同时测定了芦丁、槲皮素和连翘苷 ,研究了各种条件的影响 ,得到了优化的实验条件 ,在 2 0mmol/L的Na2 B4O7(H3BO3调节至pH 8 40 ) 30mmol/L十二烷基硫酸钠 1 0 %乙腈 ( 1 + 9)的缓冲溶液中 ,分离电压为 1 2kV时 ,芦丁、槲皮素和连翘苷在 1 0min内得到了良好的分离 ,检测波长为2 5 4nm ,芦丁、槲皮素和连翘苷分别在 0 0 1～ 1 0mg/mL ,0 0 1～ 1 0mg/mL和0 0 5～ 1 0mg/mL质量浓度范围内与电泳峰高呈现良好线性关系 ,检测下限分别为 0 0 0 5mg/mL ,0 0 0 5mg/mL和 0 0 1mg/mL 。The determination of rutin, quercetin and forsythin in traditional Chinese herbs was investigated by capillary electrophoresis. With borate buffer at 8.4 containing SDS and acetonitrile(10%) as back ground electrolyte, 12 kV applied voltage, and 254 nm as UV detection wave length, the three compounds were completely separated within 10 min. The effect of buffer pH, concentration of borate and SDS were discussed. Regression equations revealed linear relationships between the peak area of each component and the content.

关 键 词：毛细管电泳法 分离 测定 芦丁 槲皮素 连翘苷 中药 

分 类 号：R284.2[医药卫生—中药学] O657.8[医药卫生—中医学]