预防SARS病毒核酸疫苗的构建  

Construction of DNA vaccine against severe acute respiratory syndrome

作　　者：易艳萍[1] 李平[1] 李楚芳[1] 刘萱[1] 黄维[1] 靳彦文[1] 李晓荣[1] 马清钧[1] 曹诚[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100850

出　　处：《生物技术通讯》2003年第3期200-201,共2页Letters in Biotechnology

摘　　要：本研究通过反转录-PCR获得了SARS冠状病毒辐条样蛋白、核衣壳蛋白和膜蛋白基因,将所获得的可能与免疫保护相关的基因克隆至核酸疫苗表达载体pcDNA-ThyA中,酶切及序列分析结果均表明载体构建正确,该候选DNA疫苗已用于动物免疫实验。Gene encoding spike protein,nucleocaspid protein and small membrane were obtained by reverse tran-scription-polymerase chain reaction.The genes were cloned into the expression vector for DNA vaccine,and the cor-rect constructs were confirmed by restriction endonuclease digestion and DNA sequencing.The vaccine candidates have been subjected to animal inoculation to access their immunogenicity.

关 键 词：SARS病毒 核酸疫苗 核衣壳蛋白 冠状病毒 

分 类 号：R392[医药卫生—免疫学] R373.1[医药卫生—基础医学]