葡激酶及其化学修饰研究进展  

Progress in staphylokinase and its chemical modification

作　　者：熊剑[1] 董伟[1] 李勇[1] 王纲[1] 

机构地区：[1]南京理工大学化工学院,南京210094

出　　处：《生物技术通讯》2003年第3期207-209,共3页Letters in Biotechnology

摘　　要：葡激酶(SAK)是一种由金黄色葡萄球菌分泌的含有136个氨基酸残基的蛋白质,大量研究证明它具有潜在的溶血栓特性。但葡激酶是一种外源蛋白,注入体内会引起不良反应,解决这些问题的重要途径就是对蛋白质进行化学修饰。本文综述SAK的结构、溶栓机理、化学修饰及临床研究等。Staphylokinase (SAK),secreted by certain strains of Staphylococcus aureus and produced by recombinant DNA technology,is a136-amino-acid single-chain protein without disulfide bridges.It's thrombolytic potential and fibrin specificity were demonstrated only few years ago,and many clinical trials demonstrated that SAK would be a promising thrombolytic reagent.But such problems as strong immunogenicity,short circulatory half-life make it in-valid in clinical use.One way to solve these problems is to modify it with polyethylene glycol(PEG).This review in-troduced the structure and thrombolytic mechanism of SAK,SAK's peglation and clinical research concerned.

关 键 词：葡激酶 化学修饰 金黄色葡萄球菌 溶血栓特性 

分 类 号：Q55[生物学—生物化学]