人源抗严重急性呼吸综合征(SARS)病毒基因工程抗体的初步研究  

SARS Patients' Derived Human Recombinant Antibodies Recognize SARS Virus N Protein

作　　者：杜润蕾[1] 于建石[1] 梁米芳[1] 刘琴芝[1] 李川[1] 韩露露[1] 段淑敏[1] 周为民[1] 张全福[1] 王涛[1] 毕胜利[1] 李德新[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100052

出　　处：《病毒学报》2003年第2期104-108,共5页Chinese Journal of Virology

基　　金：国家"863"SARS研究重大专项资助(项目批准号:20 0 3AA2 0 82 0 9)

摘　　要：严重急性呼吸道综合征(severeacuterespiratorysyndrome,SARS)或称传染性非典型肺炎,已严重威胁人民健康和生命安全。快速研制一种可用于紧急预防SARS病毒感染的基因工程抗体预防制剂迫在眉睫。为此,运用噬菌体表面呈现技术,从多个SARS病人恢复期血中获得淋巴细胞,通过基因工程手段,构建了人源抗SARS病毒基因工程抗体文库,并筛选获得37株特异抗SARS病毒基因工程Fab抗体,其中11株人源抗体结合基因工程重组的SARS病毒核(N)蛋白,其中的1株在Westernblot分析中与SARS病毒结合,识别SARS病毒N蛋白线性位点。对所获抗体的功能鉴定及基因分析正在进行中。人源抗SARS病毒基因工程抗体的获得,将对SARS疾病的特异性预防,治疗和诊断提供新的途径。The severe acute respiratory syndrome(SARS)or so called the atypical pneumonia has seriously threatened the public health.It is urgent to develop a specific SARS virus-targeted antibody preparation for emergent prophylaxis and treatment of SARS virus infection.By using phage display technology,we constructed a naive antibody library from multiple convalescent SARS patients' lymphocytes,and 37 human Fab antibodies were selected from the library,11 of them were identified to recognize recombinant SARS N protein which was expressed and purified from E.coli.One of the SARS specific Fab antibodies was detected by Western blot,indicating the recognization of a linerized epitope of N protein.The characterization and the function canalization of the SARS patients' derived human antibodies are undergoing.The results will bring new prospect for the prevention and therapy of SARS in the future and the antibody to N protein can be used to develop clinical SARS antigen diagnositic kit.

关 键 词：严重急性呼吸综合征 SARS病毒 基因工程抗体 FAB抗体 抗体库 

分 类 号：R373.1[医药卫生—病原生物学] R392[医药卫生—基础医学]