鸡贫血病毒衣壳蛋白基因克隆、测序和比较  

Cloning and Sequencing of the Capsid Gene of Chicken Anemia Virus(CAV) of the Harbin Isolate and Its Comparison with Other CAV Strains

作　　者：何成强[1] 丁乃峥[1] 李景鹏[2] 李云龙[1] 

机构地区：[1]山东师范大学生物系动物抗性实验室,济南250014 [2]东北农业大学生命科学学院,哈尔滨150030

出　　处：《病毒学报》2003年第2期179-182,共4页Chinese Journal of Virology

基　　金：黑龙江省科委攻关项目

摘　　要：VP1 gene of chicken anemia virus in liver of infected chicken from Harbin was amplified by polymerase chain reaction and cloned into pUC18The recombinant vector was identified by restriction digestionThree clones were sequenced with sequencing kit ABI PRISM by TaKaRa CoLtdThe open reading frame of the VP1 gene is made up of 1,350bpIt encodes 449 amino acid residuesIt was found that there is an abundant Arg region from position 3 to 46Its isoelectric point is up to 107 because of its abundant basic amino acidsComparing this Harbin isolate with 26p4,Cux-1,82-2,Del-rose,A2,Connb,L-028,TR20,CIA-1,CAV15,2A9 showed there were 168 nucleoside differences which lead to 26 amino acid changes of the VP1The amino acid changes may influence the antigenic character of different VP1sIt was also found that there is a hyper-mutation region from amino acids position 139-157,its mutation frequency is up to 42% among these CAV’s VP1s.It is necessary to research the function of thisVP1 gene of chicken anemia virus in liver of infected chicken from Harbin was amplified by polymerase chain reaction and cloned into pUC18The recombinant vector was identified by restriction digestionThree clones were sequenced with sequencing kit ABI PRISM by TaKaRa CoLtdThe open reading frame of the VP1 gene is made up of 1,350bpIt encodes 449 amino acid residuesIt was found that there is an abundant Arg region from position 3 to 46Its isoelectric point is up to 107 because of its abundant basic amino acidsComparing this Harbin isolate with 26p4,Cux-1,82-2,Del-rose,A2,Connb,L-028,TR20,CIA-1,CAV15,2A9 showed there were 168 nucleoside differences which lead to 26 amino acid changes of the VP1The amino acid changes may influence the antigenic character of different VP1sIt was also found that there is a hyper-mutation region from amino acids position 139-157,its mutation frequency is up to 42% among these CAV's VP1s.It is necessary to research the function of this region

关 键 词：鸡贫血病毒 衣壳蛋白基因 克隆 测序 

分 类 号：S855.3[农业科学—临床兽医学] S858.31[农业科学—兽医学]