荧光光谱法研究肌红蛋白活性中心铁卟啉与铜离子的相互作用  

Fluorescence Spectroscopic Study of Interaction between Fe-protoporphyrin in Myoglobin and Cu(Ⅱ)Ions

作　　者：冯玉英[1] 杨辉[2] 顾晓天[1] 姜慧君[2] 陆天虹[2] 

机构地区：[1]南京师范大学分析测试中心 [2]南京师范大学化学与环境科学学院,江苏南京210097

出　　处：《光谱学与光谱分析》2003年第3期532-534,共3页Spectroscopy and Spectral Analysis

基　　金：江苏省教育厅自然科学基金资助项目 (2 0 0 0LHZOOOOSJI和 0 1KJB1 50 0 0 4 );国家自然科学基金资助项目 (2 99730 1 5)

摘　　要：首次应用荧光光谱法研究了肌红蛋白 (FePP Mb)的活性中心铁卟啉与铜离子的相互作用 ,结果表明在水溶液中肌红蛋白中的铁可被铜离子取代 ,形成铜卟啉化合物 (CuPP Mb) ,并且随着体系中铜离子量的增加 ,其相互作用增强。本课题从蛋白质的角度研究了金属离子同生物大分子相互作用的现象并探讨了作用机理 ,可以为进一步研究有害重金属在人体内作用及毒害机理提供一定的理论依据。同时 ,本文采用的荧光光谱法与常规的UV和CD法相比有较高的灵敏度。In this paper, the interaction between Cu(Ⅱ) ions and Fe-protoporphyrin in horse-heart myoglobin (FePP-Mb) was studied. As a result, some of the Fe(Ⅱ) ions in FePP-Mb were found to be replaced by Cu(Ⅱ) ions forming CuPP-Mb, by adding Cu(Ⅱ) ions into the myoglobin solution. The interaction became stronger when adding more Cu(Ⅱ) ions into the myoglobin solution. By studying the metal ions′ interaction with myoglobin proteins as macromolecules and discussing the interaction mechanism, this work provides a theoretical basis for the further study of hazardous metal ions′ interaction with the human body and its mechanism. The fluorescence spectroscopic method used in this study has higher sensitivity than the ordinary UV and CD methods.

关 键 词：荧光光谱 肌红蛋白 铁卟啉 铜离子 相互作用 重金属 毒害机理 

分 类 号：R991[医药卫生—毒理学] Q593.3[医药卫生—药学]