双嘧达莫与牛血清白蛋白结合热力学特征的荧光光谱法研究  

Study on Thermodynamics of Binding Reaction of Dipyridamole with Bovine Serum Albumin

作　　者：颜承农[1] 上官云凤[1] 童金强[1] 刘义[2] 庞代文[2] 潘祖亭[2] 屈松生[2] 

机构地区：[1]荆州师范学院化学系,湖北荆州434020 [2]武汉大学化学与分子科学学院,湖北武汉430072

出　　处：《光谱学与光谱分析》2003年第3期543-546,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金 (2 99730 30 );湖北省教育厅重点项目 (2 0 0 1A41 0 0 5)资助

摘　　要：用荧光光谱法和紫外 可见吸收光谱法研究了在不同温度下 ,双嘧达莫与牛血清白蛋白结合反应的荧光猝灭光谱行为 ,得出了结合常数、结合位点和基本热力学参数。The binding feature of dipyridamole with bovine serum albumin (BSA) was studied using fluorescence spectroscopy. It was shown that this compound has a powerful ability to quench the BSA fluorescence via a nonradiative energy transfer mechanism. The fluorescence quenching data were analyzed according to stern-volmer equation and double-reciprocal equation, and the binding constant and the thermodynamic parameters were obtained.

关 键 词：双嘧达莫 牛血清白蛋白 热力学 荧光光谱法 结合反应 高血压药物 

分 类 号：R972.4[医药卫生—药品] R914.4[医药卫生—药学]