应用套式PCR检测和区分猪传染性胃肠炎病毒和猪呼吸道冠状病毒的试验  

Application of n-PCR for Detection and Differentiation of TGE Virus and Porcine Respiratory Coronavirus

作　　者：沈海娥[1] 郭福生[2] 龚振华[2] 孙淑芳[2] 裘孝良[1] 郝勤宗[1] 

机构地区：[1]河北农业大学动物科技学院,保定071001 [2]农业部动物检疫所,青岛266032

出　　处：《中国动物检疫》2003年第7期21-23,共3页China Animal Health Inspection

摘　　要：根据猪传染性胃肠炎病毒和猪呼吸道冠状病毒的S基因核苷酸序列 ,设计合成了2对引物 ,以猪传染性胃肠炎病毒和猪呼吸道冠状病毒细胞培养物为模板 ,进行PCR特异性片段扩增 ,猪传染性胃肠炎病毒扩增片段大小为886bp,猪呼吸道冠状病毒扩增片段大小为214bp。建立的套式PCR经过特异性、敏感性试验及对临床送检样品检测 ,证明本法具有快速、特异和高度敏感的特点。Two pairs of primers were synthesized based on Sgene nucleotide sequences of transmissible qastroenteritis virus and porcine respiratory coronavirus.PCR was developed to amplify specifi squences,using TGEV and PRCV cell cultures as template.The amplified segment of TGEV gene was886bp and that of PRCV gene was214bp.Results showed that the n=pcr was rapid,specific and sensitive in detecting the clinical samples.

关 键 词：应用 套式PCR检测 猪 传染性胃肠炎病毒 呼吸道冠状病毒 试验 

分 类 号：S858.28[农业科学—临床兽医学]