赛庚啶对大鼠下丘脑-腺垂体-性腺轴和钙调蛋白基因表达的影响(英文)  

Effects of cyproheptadine on hypothalamus-pituitary-gonad axis and calmodulin gene expression in rats

作　　者：胡庆伟[1] 康白[1] 高尔[1] 李广宙[2] 李锋杰[3] 

机构地区：[1]潍坊医学院应用药理学重点实验室,山东潍坊261042 [2]潍坊医学院附属医院核医学科,山东潍坊261041 [3]潍坊医学院形态学实验室,山东潍坊261042

出　　处：《中国药理学与毒理学杂志》2003年第3期161-166,共6页Chinese Journal of Pharmacology and Toxicology

基　　金：山东省卫生厅科研基金资助项目(2001CA1CCB1 )~~

摘　　要：目的　研究赛庚啶 (Cyp)对SD大鼠生殖系统内分泌功能的影响是否有性别差异。方法　 60只SD大鼠依性别各分为 3组 ,每组 1 0只 ;分别灌胃给予生理盐水 ( 5mL·kg-1·d-1) ,Cyp( 2 .4,4.8mg·kg-1·d-1) ,共 1 4d或 2 1d。放免法测定血清黄体生成素 (LH)、促卵泡激素 (FSH)、雌二醇 (E2 )、孕酮(P)、睾酮 (T)的含量。光电镜观察促性腺激素释放激素细胞、促性腺激素细胞、间质细胞、支持细胞、黄体细胞、颗粒细胞等显微、超微结构的变化。用实时荧光定量PCR技术进行逆转录聚合酶链反应 ,琼脂糖凝胶电泳鉴定扩增产物。结果　Cyp可升高雄性大鼠血清LH、T的含量 ,而对FSH含量无明显影响。Cyp可升高雌性大鼠血清LH的水平 ,降低其FSH ,P ,E2 的含量。电镜发现 ,Cyp促进雄性大鼠分泌功能 ,使雌性大鼠内分泌细胞发生退行性改变。结论Cyp对雌性大鼠下丘脑 腺垂体 卵巢轴内分泌功能有抑制作用 ,而对雄性大鼠下丘脑 腺垂体 睾丸轴内分泌功能有促进作用 ,且使终末靶腺的内分泌细胞超微结构出现相应的变化。Cyp促进雄性大鼠睾丸钙调蛋白mRNA的表达可能与Cyp促进下丘脑 垂体AIM To study whether cyproheptadine(Cyp) affects endocrine functions in reproductive system with gender difference. METHODS Sixty SD rats were randomly distributed into 3 groups according to gender, respectively, and they were administered NS(5 mL·kg -1 ·d -1 ), Cyp 2.4, 4.8 mg·kg -1 ·d -1 accordingly by ig for 14 d or 21 d. The serum levels of luteinizing hormone(LH), follicle stimulating hormone(FSH), testosterone(T), progesterone(P), estrodiol(E 2) were measured by radio immunoassay and the ultrastructure of gonadotropin releasing hormone(GnRH) cells, gonadotropin cells, Leydig cells, Sertoli cells, luteal cells, granulocytes and so on were observed by electronmicroscopy and microscopy. The calmodulin(CaM) mRNA expression in hypothalamus pituitary testis axis(HPTA) was detected by reverse transcriptase polymerase chain reaction. RESULTS Cyp(2.4 mg·kg -1 ·d -1 ×14 d, ig) increased serum LH concentration while decreased serum FSH, P concentrations in female rats. Cyp(4.8 mg· kg -1 ·d -1 ×14 d, ig)increased serum LH, T concentrations in males, and increased serum LH concentration while decreased serum FSH, E 2 and P concentrations significantly in females. The retrograde changes of ultrastructure were observed in part of gonadotropin and ovary endocrine cells, while a stimulating one in testis endocrine cells. CaM mRNA expression levels were elevated in testis but not in hypothalamus and pituitary in male rats. CONCLUSION Cyp had a negative effect on endocrine function in females, but a positive one in males. The ultrastructure showed relevant changes in target gland. Cyp promoted CaM mRNA expression in testis, Received date: 2002 07 23 Accepted date: 2002 12 20 Foundation item: The project supported by Medical Science Foundation of Provincial Health Bureau of Shandong Province(2001CA1CCB1) Biographies: HU Qing Wei(1976-), male, native of Binzhou, Shandong Province, postgraduate; KANG Bai(1955-),female, native of Weifang, Shandong Pr

关 键 词：赛庚啶 下丘脑-垂体系统 钙调蛋白 性腺 

分 类 号：R965[医药卫生—药理学]