抗氯霉素多克隆抗体的制备  

STUDY OF THE POLYCLONAL ANTIBODY AGAINST CHLORAMPHENICOL

作　　者：王谦[1] 齐孟文[1] 何方洋[1] 杨根海[1] 

机构地区：[1]中国农业大学,北京100094

出　　处：《核农学报》2003年第3期196-198,195,共4页Journal of Nuclear Agricultural Sciences

摘　　要：用重氮化和CDI两种方法合成了CAP全抗原 ,紫外分析和商业试剂盒ELISA鉴定表明合成成功 ;动物免疫 4次后采血检测 ,多克隆抗体 50 %抑制率探测限可达1 0ng/ml,效价可达 1 0万以上稀释倍数 ,特异性较好。The antigen of Chloramphenicol was synthesized by CDI reaction and diazotization reaction respectively,and identified with UR scan and imported commercial kit.After immunized 4 times,the sensitivity of the antiserum obtained was more than 10ng/ml.

关 键 词：氯霉素 抗氯霉素多克隆抗体 制备 重氮化 CDI CAP全抗原 合成 碳二亚胺 ELISA试剂盒 药物残留 

分 类 号：S859.797[农业科学—临床兽医学]