rhNDPK-α蛋白对正常小鼠脾细胞增殖和细胞周期的影响  

Effect of rhNDPK-α protein on proliferation and cell cycle of normal mice splee n lymphocytes

作　　者：李海燕[1] 王一飞[2] 张美英[2] 刘洁生[1] 

机构地区：[1]暨南大学生物工程学系,广东广州510632 [2]暨南大学生物医药开发研究基地,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2003年第3期98-102,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家十五"863"项目(2001AA215041);广东省科委重大攻关项目(A1090207);广州市科委重大专项(99-Z-007-01).

摘　　要：　目的:探讨rhNDPK-α蛋白对正常小鼠脾细胞增殖和细胞周期的影响.方法:不同质量浓度的rhNDPK-α蛋白体外作用于脾细胞,通过MTT法和流式细胞术测其增殖和细胞周期.结果:rhNDPK-α蛋白的质量浓度为10～250μg·mL-1时对脾细胞的自发增殖反应有明显的促进作用;10～50μg·mL-1时对PHA诱导的脾细胞增殖有明显的协同作用;50μg·mL-1rhNDPK-α蛋白作用小鼠脾细胞24h后,G0/G1和G2-M期细胞比例明显增加,而S期比例显著减少.结论:rhNDPK-α蛋白可促进脾细胞的存活,很可能通过调控细胞周期而参与细胞的生长调节.Aim: To evaluate the effect of rhNDPK-α protein on proliferation and cell cycle of normal mice spleen lymphocytes in this article. Me thods: T he different concentration of rhNDPK-α is added into the spleen lymphocytes i n vitro, through the assay of MTT and FCM, the proliferation and cell cycle are de tected. Results: Certain concentration of rhNDPK-α protein can promote the spon taneous proliferaton of spleen cell, synergize the induced proliferation by PHA and change the cell cycle. Conclusion: rhNDPK-α protein can pro mote the surviva l of spleen cell and may take part in the adjustment of cell growth by the way o f changing cell cycle. 

关 键 词：重组人NDPK-α 流式细胞术 细胞周期 

分 类 号：R392.12[医药卫生—免疫学] Q253[医药卫生—基础医学]