血清性激素在白腰文鸟前脑发声控制核团神经元早期性双态分化中的作用  

Effects of serum sex steroid on the early neuronal dimorphism in forebrain vocal-control nuclei of songbird (Lonchura striata)

作　　者：曾少举[1] 张信文[2] 李雪生[1] 左明雪[1] 

机构地区：[1]北京师范大学生命科学学院,北京100875 [2]海南师范学院生物系,海口市571158

出　　处：《动物学报》2003年第3期353-361,共9页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金资助项目 (No :39870 0 97; 30 0 6 0 0 2 7和 30 2 70 449)~~

摘　　要：本文究了雌、雄白腰文鸟 (Lonchurastriataswinhoei)不同发育时期前脑四个控制发声重要核团古纹状体栎核 (RA)、新纹状体前部巨细胞核外侧部 (LMAN)、X区 (AreaX)和高级发声中枢 (HVC)中神经元数量、体积和体内雌二醇 (E2 )和睾酮 (T)浓度的变化 ,以揭示性激素对鸣禽发声核团性双态性分化的影响。结果发现 :( 1)HVC、LMAN和X区在发育早期神经元数量和体积均呈显著性双态性差异 ,而RA神经元直至 30日龄 (P30 )后才显示出明显性别差异 (P <0 0 5 ) ;( 2 )除RA外 ,HVC、LMAN和X区神经元体积的显著性双态性差异均发生在P2 0左右 ,P2 0后雌、雄核团内的神经元体积仅有较小范围的波动 ;( 3)RA和LMAN神经元数量随年龄增长而逐渐减少 ;雌、雄鸟HVC和雄鸟X区的神经元数量在P2 0～ 30间均增长 ,雄鸟HVC的增长幅度显著大于雌鸟。P30后HVC和X区的神经元数量不再增加 ,开始小幅度减少 ;( 4)四个发声核团的神经元数量和体积在P5～ 12 0期间均出现 1～ 2个急剧变化期 ,此变化期与体内雌激素水平开始出现显著性差异的临界期及核团间神经联系开始建立的时期相对应 ;( 5 )雌、雄鸟血清中E2 的水平在核团发育初期 (P5 )差异显著 ,雌鸟为雄鸟的 7 45倍 ,P5后则呈相反方向变化趋势 ,在P15时雄鸟中的E2 水平反超过雌鸟 ,差异?We investigated the changes of neuronal numbers and neuronal sizes of four control-vocal nuclei: robust nucleus of the archistriatum (RA), lateral magnocellular nucleus of the anterior neostriatum(LMAN), Area X and high vocal center (HVC) from posthatching days (P) P5 to P120 in the songbird , striated mannikin (Lonchura striata)by use of histological method of nissl-staining. The serum estrodial concentrations from P5 to P50 and the serum testosterone concentrations from P5 to P120 were also tested to further understand the endocrine mechanism of sexual differences in the song control nuclei. The results were as follows:(1)The numbers of RA neuron were not significantly different between males and females before P30. The numbers of RA neuron decreased most significantly from P30 to P40, approximately corresponding to the time when the neural connections were established between RA and HVC. The neuronal numbers of RA in females decreased much more rapidly than those in males during P20 and P30, leading to the significant differences of RA neuronal number between males and females (P<0.05). The numbers of LMAN neurons exhibited sexually significant differences even in P15. The numbers of LMAN neurons in both males and females decreased significantly only in two adjacent groups at P20 and P40. These two periods were approximately corresponded to the time when neural connections were formed between LMAN and RA or between HVC and RA respectively. The numbers of female area X neurons in males increased most significantly during P20 and P30, but they did not change significantly after P30. The outline of Area X in females could not be clearly distinguished, so the neuronal numbers of female Area X were not available in this study. The numbers of HVC neurons continued to increase before P30, but they increased no more after P30 both in males and females. The decrease of HVC numbers was most obvious in the period from P30 to P40 for both sexes. (2)The volumes of RA, LMAN, Area X or HVC neurons continued to increase be

关 键 词：血清性激素 白腰文鸟 前脑发声控制核团 神经元 性双态分化 雌二醇 睾酮 

分 类 号：Q424[生物学—神经生物学]