扬子鳄的CaSox4基因的分子克隆和进化分析  

Molecular cloning and evolution analysis of the CaSox4 gene of Alligator sinensis

作　　者：郑济芳[1] 朱睦元[1] 

机构地区：[1]浙江大学生命科学学院遗传研究所,杭州310012

出　　处：《动物学报》2003年第3期404-407,共4页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金 (No 39770 42 0和No 30 10 0 115 )资助项目~~

摘　　要：The completely identical HMG-box motif of CaSox4 gene from both male and female genomic DNA of the Chinese alligator (Alligator sinensis) was cloned and sequenced by degenerate primer PCR. Compared with the human and mouse SRY, CaSox4 revealed 51% and 57% nucleotide homology respectively and 49% and 55% amino acid identity respectively. CaSox4 belongs to subgroup C of the Sox gene family. The GC content is 86% in the HMG-box region of the CaSox4 gene. Blast analysis showed that the CaSox4 gene shares 100% amino acid identity with human Sox4, bird SoxLF4, turtle Sra4 and lizard CvSox4 genes. CaSox4 may be orthologous with the human Sox4 gene. This indicates that CaSox4 gene shows the remarkable evolutionary conservation during the evolution of Alligator sinensis. The extensive sequence conservation of the Sox4 gene between reptiles, mammals and birds suggests major functional constraints.The completely identical HMG-box motif of CaSox4 gene from both male and female genomic DNA of the Chinese alligator (Alligator sinensis) was cloned and sequenced by degenerate primer PCR. Compared with the human and mouse SRY, CaSox4 revealed 51% and 57% nucleotide homology respectively and 49% and 55% amino acid identity respectively. CaSox4 belongs to subgroup C of the Sox gene family. The GC content is 86% in the HMG-box region of the CaSox4 gene. Blast analysis showed that the CaSox4 gene shares 100% amino acid identity with human Sox4, bird SoxLF4, turtle Sra4 and lizard CvSox4 genes. CaSox4 may be orthologous with the human Sox4 gene. This indicates that CaSox4 gene shows the remarkable evolutionary conservation during the evolution of Alligator sinensis. The extensive sequence conservation of the Sox4 gene between reptiles, mammals and birds suggests major functional constraints.

关 键 词：扬子鳄 CaSox4基因 分子克隆 进化 

分 类 号：Q785[生物学—分子生物学]