玉米候选抗病相关基因片段的克隆  

Cloning of the DNA Fragment of Related Resistance Gene in Maize

作　　者：翟淑梅[1] 孟辉[1] 尹小燕[1] 张举仁[1] 

机构地区：[1]山东大学生命科学学院,山东济南250100

出　　处：《山东大学学报（理学版）》2003年第2期97-100,104,共5页Journal of Shandong University(Natural Science)

基　　金：国家转基因植物研究与产业化项目(J0 0 -A -0 0 2 );国家高技术研究资助项目 (2 0 0 1AA2 11111)

摘　　要：根据已经克隆的植物抗病基因和候选抗病基因的保守序列P -loop、Kinase - 2及GLPLAL设计一系列简并引物 ,利用同源序列扩增法 ,对玉米的基因组DNA进行PCR扩增 ,并对 5个扩增产物的克隆进行测序 .测序结果在Gen Bank内进行BLAST检索 ,发现A9克隆序列与玉米BAC库中的 2 0 6C17克隆的部分序列有很高的相似性 ,并且距离GenBank内注册的玉米抗锈病基因rp1位点中的rp1- 3基因、rp1- 4基因分别约有 6 6Kb、2 0Kb ,且A9克隆序列在玉米基因组中是单拷贝的 .这为玉米抗锈病性状的分子标记辅助选择和抗锈病基因的克隆奠定了良好的基础 .A series of degenerate primers based on conserved motifs P loop?kinase 2?GLPLAL of known plant resistance gene proteins and candidate resistance gene proteins were used to amplify sequences from Maize genomic DNA. Several amplified DNA fragments were cloned and five among them were sequenced. Compared with the registered genes in GenBank, a 259bp A9 clone has 98% similarity with the Maize BAC clone 206C17(99156bp long) at 98332-98461bp. This site was only about 20Kb from resistance gene rp1-4 and 66Kb from rp1-3. So it provides a sound basis for the isolation of the rust resistance genes and marker-assisted selection for the rust resistant trait in maize.

关 键 词：玉米 抗病基因 简并引物 克隆 

分 类 号：Q781[生物学—分子生物学]