vvIBDV诱导幼鸡免疫细胞凋亡的研究  

Apoptosis of Chicken Immunocytes Induced by vvIBDV

作　　者：张燕[1] 赵晖[1] 任前升[1] 毛炳宇[1] 王振光[1] 张红卫[1] 

机构地区：[1]山东大学生命科学学院发育生物学研究所,山东济南250100

出　　处：《山东大学学报（理学版）》2003年第2期105-107,111,共4页Journal of Shandong University(Natural Science)

基　　金：国家自然科学基金 (3 0 0 70 0 94)支持

摘　　要：为研究超强毒传染性法氏囊病病毒 (vvIBDV)感染的致病机理 ,在 3— 6周龄SPF幼鸡法氏囊、脾脏、胸腺细胞培养体系中加入vvIBDV ,以流式细胞术 (FcM)、DNA琼脂糖凝胶电泳技术检测vvIBDV诱导的细胞凋亡 ,结果表明 ,在本实验的体外培养体系中 ,vvIBDV可直接和迅速引起幼鸡法氏囊细胞凋亡 。To study the pathopoiesis of very virulent infectious bursal disease(vvIBDV),we studied the apoptosis of cultured bursa,spleen and thymus cells obtained from freshly isolated tissues of 3 6 week old SPF chickens cultured with vvIBDV. Apoptosis rate was analyzed by flow cytometry (FcM) and apoptosis was detected by agarose gel electrophoresis of extracted genomic DNA,Results showed that vvIBDV could induce apoptosis of 3 6 week old SPF chicken's bursa cells cultured in vitro,but had no apparent apoptosis inducing effect on spleen or thymus cells.

关 键 词：VVIBDV 免疫细胞 细胞凋亡 细胞培养 

分 类 号：Q255[生物学—细胞生物学]