应用高效液相色谱法对兔玻璃体中盐酸川芎嗪含量的测定  

Determination of hydrochloric ligustrazine in rabbit vitreous with high performance liquid chromatography

作　　者：薛淑红[1] 柯雪红[2] 王燕[1] 沈兰芳[1] 余杨桂[1] 李志英[1] 

机构地区：[1]广州中医药大学第一附属医院眼科,广州5104053 [2]广州中医药大学第一附属医院实验中心

出　　处：《中国中医眼科杂志》2003年第2期69-71,共3页China Journal of Chinese Ophthalmology

基　　金：广东省教育厅自然科学研究项目 (2 0 0 0 74 )

摘　　要：目的　了解高效液相色谱 (highperformanceliquidchromatography,HPLC)技术在兔直流电盐酸川芎嗪注射液离子导入后在测定玻璃体盐酸川芎嗪含量方面的应用。方法　色谱条件是色谱柱为ODSC1 8不锈钢柱 (1 50mm× 4 6mm ,5μm) ;流动相为甲醇∶水 =40∶60 ,1 0 0 0ml中含十二烷基磺酸钠 (SDS) 1g ,磷酸二氢钾 0 6g,冰醋酸 1ml;流速为 1 0ml·min- 1 ;检测波长为2 80nm ;室温操作。结果　在 0 54～ 1 7 2 8μg·ml- 1 范围内 ,峰面积与浓度呈良好线性关系 ,r =0 9994;最低检测浓度为 0 0 8μg·ml- 1 ;平均回收率为 92 542 % ;日内和日间精密度均小于 1 0 %。结论　建立专属、简单、快速和精确的高效液相色谱法可用于对盐酸川芎嗪在兔玻璃体内药代动力学的研究。OBJECTIVE To assess application of application of high performance liquid chromatography(HPLC)for determination of hydrochloric ligustrazine in rabbit vitreous after transmitting the ion of injection of ligustrazine into vitreous. METHODS The analytical column was a ODS C 18 stainless steel column(150mm×4.6mm, 5μm). The mobile phase consists of 40 percent methanol and 60 percent H 2O. In 1000ml mixture of the mobile phase there are 1.0g SDS, 0.6g KH 2PO 4 and 1ml glacial acetic acid.The flow rate is 1.0ml·min -1 . The wavelength of the detector is 280nm. The operation is carried in room temperature.RESULTS The relationship between the peak area and concentration of the drug is linear within the range of 0.54～17.28μg·ml -1 with a correlation,r=0.9994. The minimal detectable limit was 0.08μg·ml -1 . The average recovery is 92.542 percent. RSD of intra-day and inter-day are all lower than 10%. CONCLUSION HPLC is a specific,simple,rapid,accurate method, and the suitable method established could be applied for the pharmacokinetic research of hydrochloric ligustrazine in rabbit vitreous.

关 键 词：川芎嗪 高效液相色谱 玻璃体 

分 类 号：R969.1[医药卫生—药理学] R284.1[医药卫生—药学]