以丙型肝炎病毒体内感染裸鼠模型筛选20种常用清热解毒类中药  

Screening 20 Chinese Herbs often Used for Clearing Heat and Dissipating Toxin with Nude Mice Model of Hepatitis C Viral Infection

作　　者：唐智敏[1] 彭萌[2] 詹春姣[3] 李航森[2] 石淑仙[3] 

机构地区：[1]广东省中医院内科,广州510120 [2]湖北中医学院附属医院 [3]华中科技大学同济医学院附属同济医院

出　　处：《中国中西医结合杂志》2003年第6期447-448,共2页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金(No.39670910)

摘　　要：目的:用HCV体内感染裸鼠模型筛选20种常用清热解毒类中药,以寻找有效的抗HCV药物。方法:模型鼠用药3个月,用透射电子显微镜观察裸鼠脾内移植的人胎肝细胞中是否仍有HCV样颗粒,用定量RT-PCR技术检测用药前后血清HCVRNA含量。结果:(1)各中药组,用药3个月在裸鼠脾脏内移植的人胎肝细胞中均可找到HCV样颗粒。(2)仅龙胆草、黄芩、山豆根、栀子、苦参5味中药组,用药3个月后血清HCVRNA含量明显下降,其他中药组,用药3个月前后血清HCVRNA含量无明显下降。结论:该20种中药均无直接清除HCV的作用,但龙胆草、黄芩、山豆根、栀子及苦参5味中药可明显抑制HCVRNA的复制。Objective: To seek effective drugs for anti-hepatitis C virus by screening 20 Chinese herbs often used for clearing heat and dissipating toxin with nude mice model of hepatitis C viral (HCV) infection. Methods: After the model mice had been treated with selected drug for 3 months, transmission electron microscope was used to observe whether the HCV-like particles in human fetal hepatocytes (HFH) transplanted into mice spleen still existed, and quantitative RT-PRC technique was used to detect the serum content of HCV-RNA before and after treatment. Results: (1)HCV-like particles existed in all the model mice after treatment. (2) Serum content of HCV-RNA decreased after treated with Radix Gentianae, Radix Scutellariae, Radix Sophorae tonkinensis, Fructus Gardeniae and Fructus Sophorae flavoscentis, but unchanged after treatment with other drugs. Conclusion: All the 20 herbs screened has not effect in directly eradicating HCV, but Radix Gentianae, Radix Scutellariae, Radix Sophorae tonkinensis, Fructus Gardeniae and Fructus Sophorae flavoscentis could significantly inhibit the replication of HCV-RNA.

关 键 词：丙型肝炎 清热解毒中药 RNA复制 RT-PCR技术 动物实验 

分 类 号：R285[医药卫生—中药学]