大鼠灌服复方丹参方后血清中丹参素的测定  

Determination of Danshensu in Rat Serum after Oral Administer Compound Salvia Recipe

作　　者：潘桂湘[1] 张伯礼[1] 高秀梅[1] 

机构地区：[1]天津中医学院中医药研究中心,天津300193

出　　处：《中国中药杂志》2003年第6期562-565,共4页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究规划项目 (G199944 0 5 44 0 3 )

摘　　要：目的 :了解大鼠ig复方丹参方后水溶性成分的吸收情况 ,建立大鼠血清中丹参素的含量测定方法。 方法 :采用液液萃取法对血清样品进行预处理 ,以对羟基苯甲酸为内标 ,甲醇 乙腈 0 .5 %醋酸 (2 .5∶3.5∶94 )为流动相 ,用高效液相色谱法于 2 81nm检测。结果 :大鼠ig复方丹参方后在血清中检测到丹参素 ,用所建立的方法绘制了其经时血药浓度曲线 ;而原儿茶醛则未在血清中发现 ,检测到的是一个保留时间稍前于原儿茶醛的未知化合物。结论 :大鼠灌服复方丹参方后 ,丹参素可经胃肠道吸收入血 ,呈快吸收慢消除的过程。Objective: To investigate the absorption of the water soluble components of Salvia miltiorrhiza and establish methods to determine Danshensu in rat serum after ig compound Salvia recipe. Method: Used liquid-liquid extraction to deal with the serum, then determined the sample by high-performance liquid chromatography. The conditions for HPLC were as the following: the mobile phase, CH_3OH-CH_3CN-0.5%CH_3COOH (2.5∶3.5∶94) with a flow rate of 1.0 mL·min -1 ; detection wavelength, 281nm; internal standard, 4-hydroxy-benzoic acid. Result: Danshensu and an unknown compound whose reserved time was little shorter than protocatechuic aldehyde were found in the rat serum after ig compound Salvia recipe, while protocatechuic aldehyde weren't found in the serum. Conclusion: Danshensu can be absorbed into blood after ig compound Salvia recipe.

关 键 词：复方丹参方 中药 丹参素 血药浓度 高效液相色谱法 动物实验 

分 类 号：R285[医药卫生—中药学]